重组Anti-Vitamin D2+Vitamin D3抗体[EPPTX-6-271-12] - BSA and Azide free
Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free
- RabMAb
- Recombinant
- 了解详情
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(1 Publication)
Rabbit Recombinant Monoclonal Ergocalciferol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.
查看别名
Ergocalciferol
- cELISA
Supplier Data
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)
ab229822 was used at 11.8 ng/ml.
The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
- cELISA
Supplier Data
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
反应性数据
产品详情
ab237700 is the carrier-free version of ab229822.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
产品实验方案
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- Visit the Troubleshooting
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
The Journal of biological chemistry 301:108250 PubMed39894217
2025
Applications
Unspecified application
Species
Unspecified reactive species
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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