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AB237700

重组Anti-Vitamin D2+Vitamin D3抗体[EPPTX-6-271-12] - BSA and Azide free

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Ergocalciferol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

查看别名

Ergocalciferol

2 Images
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)

ab229822 was used at 11.8 ng/ml.

The results show cross-reactivity with Vitamin D2.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] - BSA and Azide free (AB237700)

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 50-60 ng/mL of ab229822 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPPTX-6-271-12

亚型

IgG

不含载体蛋白

Yes

应用

cELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p>Use at 50-60 ng/ml.</p>" } } }

产品详情

ab237700 is the carrier-free version of ab229822.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

See full target information Ergocalciferol

其他靶点

Vitamin D3

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108250 PubMed39894217

2025

Modulating the complement system through epitope-specific inhibition by complement C3 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhidong Chen,Mingshuang Wang,Wenqian Duan,Yi Xia,Huiqin Liu,Feng Qian
View all publications

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