Anti-Vitamin D Receptor抗体(ab3508)
Key features and details
- Rabbit polyclonal to Vitamin D Receptor
- Suitable for: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
概述
-
产品名称
Anti-Vitamin D Receptor抗体
参阅全部 Vitamin D Receptor 一抗 -
描述
兔多克隆抗体to Vitamin D Receptor -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Chicken, Human
预测可用于: Cow, Pig, Zebrafish, Saguinus oedipus
-
免疫原
Synthetic peptide corresponding to Human Vitamin D Receptor aa 350-450.
Database link: P11473 -
阳性对照
- IHC: Human jejunum
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| EMSA |
Use at an assay dependent concentration.
|
|
| Gel Shift Assay |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ChIP |
Use at an assay dependent concentration. PubMed: 17244627
|
|
| WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P | (1) |
1/2000 - 1/4000.
|
| ChIP-sequencing |
Use at an assay dependent concentration. PubMed: 21846776
|
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
EMSA
Use at an assay dependent concentration. |
|
Gel Shift Assay
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ChIP
Use at an assay dependent concentration. PubMed: 17244627 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
1/2000 - 1/4000. |
|
ChIP-sequencing
Use at an assay dependent concentration. PubMed: 21846776 |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
-
功能
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. -
疾病相关
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all -
别名
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
- 1,25 dihydroxyvitamin D3 receptor antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)
Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
Left panel: with primary antibody at 1/2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (73)
ab3508 被引用在 73 文献中.
- Wang Q et al. Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription. J Bone Miner Res 38:427-442 (2023). PubMed: 36625422
- Chen J et al. 1,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice. Int J Biol Sci 19:610-624 (2023). PubMed: 36632467
- Jiao S et al. Calcipotriol Attenuates Form Deprivation Myopia Through a Signaling Pathway Parallel to TGF-β2-Induced Increases in Collagen Expression. Invest Ophthalmol Vis Sci 64:2 (2023). PubMed: 36723926
- Koll L et al. Exploiting Vitamin D Receptor and Its Ligands to Target Squamous Cell Carcinomas of the Head and Neck. Int J Mol Sci 24:N/A (2023). PubMed: 36902107
- Han YC et al. VDR alleviates endothelial cell injury in arteriovenous fistula through inhibition of P66Shc-mediated mitochondrial ROS. Sci Rep 13:11088 (2023). PubMed: 37422508