为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Vitamin D Receptor抗体(ab3508)

  • Datasheet
  • SDS
Reviews (6)Q&A (3)References (51)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)

    Key features and details

    • Rabbit polyclonal to Vitamin D Receptor
    • Suitable for: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IF
    • Reacts with: Mouse, Rat, Chicken, Human
    • Isotype: IgG

    你可能会感兴趣

    一抗
    Product image
    Anti-PAX5 antibody (ab15164)
    二抗
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    一抗
    Product image
    Anti-CYP27B1 antibody [EPR20271] (ab206655)

    查看更多相关产品

    概述

    • 产品名称

      Anti-Vitamin D Receptor抗体
      参阅全部 Vitamin D Receptor 一抗
    • 描述

      兔多克隆抗体to Vitamin D Receptor
    • 宿主

      Rabbit
    • 经测试应用

      适用于: IHC-Fr, ChIP, WB, IP, IHC-P, ChIP-sequencing, ICC/IFmore details
    • 种属反应性

      与反应: Mouse, Rat, Chicken, Human
      预测可用于: Cow, Pig, Zebrafish, Saguinus oedipus
    • 免疫原

      Synthetic peptide corresponding to Human Vitamin D Receptor aa 350-450.
      Database link: P11473

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • IHC: Human jejunum
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Vitamin D Receptor
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Vitamin D Receptor
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Other
      • Neuroscience
      • Development
      • Neuroscience
      • Processes

    相关产品

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Vitamin D Receptor protein (ab82068)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3508于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    EMSA
    Use at an assay dependent concentration.
    Gel Shift Assay
    Use at an assay dependent concentration.
    IHC-Fr (1)
    Use at an assay dependent concentration.
    ChIP
    Use at an assay dependent concentration. PubMed: 17244627
    WB (3)
    Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.

    1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).

    IP
    Use at an assay dependent concentration.
    IHC-P (1)
    1/2000 - 1/4000.
    ChIP-sequencing
    Use at an assay dependent concentration. PubMed: 21846776
    ICC/IF (1)
    Use at an assay dependent concentration.
    说明
    EMSA
    Use at an assay dependent concentration.
    Gel Shift Assay
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ChIP
    Use at an assay dependent concentration. PubMed: 17244627
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.

    1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).

    IP
    Use at an assay dependent concentration.
    IHC-P
    1/2000 - 1/4000.
    ChIP-sequencing
    Use at an assay dependent concentration. PubMed: 21846776
    ICC/IF
    Use at an assay dependent concentration.

    靶标

    • 功能

      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
    • 疾病相关

      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
    • 序列相似性

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession P11473 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7421 Human
      • Entrez Gene: 22337 Mouse
      • Entrez Gene: 24873 Rat
      • Omim: 601769 Human
      • SwissProt: P11473 Human
      • SwissProt: P48281 Mouse
      • SwissProt: P13053 Rat
      • Unigene: 524368 Human
      • Unigene: 245084 Mouse
      • Unigene: 10911 Rat
      see all
    • 别名

      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)

      Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
      Left panel: with primary antibody at 1/2000. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (51)

    发表研究结果有使用 ab3508?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3508 被引用在 51 文献中.

    • Lin J  et al. Upregulation of microRNA-181a-5p increases the sensitivity of HS578T breast cancer cells to cisplatin by inducing vitamin D receptor-mediated cell autophagy. Oncol Lett 21:247 (2021). PubMed: 33664811
    • Qin LY  et al. The combination of vitamin D3 and erythropoietin alleviates acute kidney injury induced by ischemia-reperfusion via inhibiting inflammation and apoptosis. Iran J Basic Med Sci 24:167-174 (2021). PubMed: 33953855
    • Kim H  et al. Brain Endothelial P-Glycoprotein Level Is Reduced in Parkinson's Disease via a Vitamin D Receptor-Dependent Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 33198348
    • Zhang H  et al. Vitamin D receptor targets hepatocyte nuclear factor 4a and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem 295:3891-3905 (2020). PubMed: 32051143
    • Wang D  et al. 1a,25-Dihydroxyvitamin D3 prevents renal oxidative damage via the PARP1/SIRT1/NOX4 pathway in Zucker diabetic fatty rats. Am J Physiol Endocrinol Metab 318:E343-E356 (2020). PubMed: 31891537
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
    Specification
    NIH/3T3, untransfected, mVDR-transfected
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. A Uhmann

    Verified customer

    提交于 Dec 03 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vitamin D Receptor antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (oral mucosa)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    oral mucosa
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    PROF. Ming-Yi Shen

    Verified customer

    提交于 Aug 20 2021

    Western blot abreview for Anti-Vitamin D Receptor antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hodgkin lymphoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Hodgkin lymphoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 02 2020

    Western blot abreview for Anti-Vitamin D Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels)
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Daniel Tan

    Verified customer

    提交于 Oct 28 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Whole embryo)
    Specification
    Whole embryo
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 14 2009

    Western blot abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (NIH/3T3, untransfected, VDR-transfected)
    Loading amount
    10 µg
    Specification
    NIH/3T3, untransfected, VDR-transfected
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. A Uhmann

    Verified customer

    提交于 Dec 01 2009

    Question

    I recently ordered Anti Vitamin D receptor antibody – ChIP grade ab3508, on the data sheet it says concentration not determined. The lot number is GR41724-6. Do you happen to know the concentration, and do you have any recommendations for a protocol? I have used the antibody at a 1/2000 1/4000 dilution (if the ab is 1mg/ml) and I get really high background staining with low nuclear staining. I use citrate buffer pH 6.0 for HIER for 40 minutes in a vegetable steamer then 30 min room temperature cool down. I use peroxidase block 10 min with 3%h2o2 and 15 min protein block using vector animal free blocker. Then I incubate the primary antibody for 30 minutes, do another protein block for 15 minutes and use MACH 4 biocare polymer for 30 minutes. Also I have tried to incubate the primary overnight and use a rabbit alkaline phosphatase secondary antibody with Vector red chromogen and I get no staining. Thus is there anything you can recommend to assist my VDR stain efforts? Thanks for your time and have a great day.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2012

    Answer

    Thank you for contacting us.

    Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and estimate 5 mg/ml).

    You may want to remove the additional protein blocking step after the primary antibody incubation. Plus he antigen retrieval step may be a bit too long. Typically 10 mins boiling in a microwave followed by 20 min cooling works well. Also, since this is a nuclear antigen, you should permeabilize with Triton X-100 if you're not already. What species are your samples from and what is the tissue type (i.e. rat spleen)?

    I'm listing some references below which have cited the use of this antibody in IHC:

    Arch Biochem Biophys. 2010 Feb 15;494(2):166-77.
    "Identification of a highly specific and versatile vitamin D receptor antibody."
    Author(s): Wang Y, Becklund BR, DeLuca HF
    Number of Citations: 1

    Biogerontology. 2008 Apr;9(2):109-18.
    "Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions."
    Author(s): González Pardo V, Boland R, de Boland AR
    Number of Citations: 1

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 13 2012

    Question

    Inquiry: Hi, I was wondering if a concentration is available for this antibody. If not, do you have any specific recommendations as to how much should be used in for a ChIP experiment? Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you very much for contacting us with your enquiry.

    Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in 4 known publications, though it's not tested in ChIP in our own lab. I've looked through the references (attached) and in "Ab3508 ChIP 1" and the protocol references of "Ab3508 ChIP 2, the authors used 5 uL of ab3508 per chromatin pellet.

    I am sorry that we don't have more information, but I hope this will be useful. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    回复于 Oct 17 2012

    Question

    What dilution was used with this antibody in IP?

    Read More

    Abcam community

    Verified customer

    Asked on May 16 2006

    Answer

    Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 May 18 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有