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Anti-Vitamin D Binding蛋白抗体[2G2] (ab23480)

Submit a review Q&A (2)References (3)

Key features and details

  • Mouse monoclonal [2G2] to Vitamin D Binding protein
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Vitamin D Binding蛋白抗体[2G2]
    参阅全部 Vitamin D Binding protein 一抗

  • 描述

    小鼠单克隆抗体[2G2] to Vitamin D Binding蛋白

  • 宿主

    Mouse

  • 特异性

    ab23480 is specific for Gc-globulin (vitamin D binding protein).

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native Gc-globulin isolated from human plasma

  • 阳性对照

    • Human plasma can be used as a positive control. The concentration of Gc-globulin in human plasma is approximately 400 µg/ml.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab23480于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/20000.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa.

(Note: In Western blotting after SDS-PAGE, HYB 249-01 reacts with Gc-globulin in both reduced as well as unreduced forms.)
说明
ELISA
1/20000.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa.

(Note: In Western blotting after SDS-PAGE, HYB 249-01 reacts with Gc-globulin in both reduced as well as unreduced forms.)

靶标

  • 功能

    Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

  • 序列相似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    文献 (3)

    发表研究结果有使用 ab23480？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab23480 被引用在 3 文献中.

    • Luebbering N  et al. Endothelial injury, F-actin and vitamin-D binding protein after hematopoietic stem cell transplant and association with clinical outcomes. Haematologica 106:1321-1329 (2021). PubMed: 32241849
    • Pottiez G  et al. Plasma proteomic profiling in HIV-1 infected methamphetamine abusers. PLoS One 7:e31031 (2012). WB ; Human . PubMed: 22359561
    • Bai S  et al. Proteome analysis of biomarkers in the cerebrospinal fluid of neuromyelitis optica patients. Mol Vis 15:1638-48 (2009). WB ; Human . PubMed: 19710940

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Dear technical team,

    I purchased ab23480, ab121060, ab90920 and would like to do sandwich ELISA.
    I would like to know epitope sequences of ab23480 and ab121060. In addition, May I ask if ab23480 can be used as capture antibody and ab121063 (instead of ab121060) as detection antibody?

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    Answer

    Thank you for contacting us.

    Full length vitamin D binding protein is used as immunogen for ab121060, ab121063 and ab23480 but we are sorry to tell that we do not have a specific epitope mapping, down to the amino acid sequence, available. These antibodies have been tested in inhibition ELISA experiment, none of the antibodies inhibit each other. Hence, we believe that they do all react with different epitopes. However, as we have not tested ab23480 in sandwich ELISA with ab121063, we cannot tell whether ab23480 can be a suitable sandwich ELISA pair with ab121063, of which you have to test in your own experiment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any further advice or information

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    Question

    We would like to purchase the positive control of this antibody. Could you please check if you have this product? https://www.abcam.com/index.html?pageconfig=datasheet&intAbID=23480 Thank you!

    Read More
    Answer

    Thank you for your enquiry. Further to correspondence with the source of this antibody I can tell you that normal human plasma should be suitable as a positive control. The concentration of Gc-globulin in human plasma is approximately 400 µg/ml (Masuda S, Okano T, Osawa K, Shinjo M, Suematsu T, Kobayashi T (1989) Concentrations of vitamin D-binding protein and vitamin D metabolites in plasma of patients with liver cirrosis. J Nutr Sci Vitaminol 35:225-34.). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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