Anti-Vitamin D Binding蛋白抗体[19E8] (ab23485)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)](https://www.abcam.cn/ps/products/23/ab23485/Images/Vitamin-D-Binding-protein-Primary-antibodies-ab23485-1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)")
Key features and details
- Mouse monoclonal [19E8] to Vitamin D Binding protein
- Suitable for: ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG2
概述
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产品名称
Anti-Vitamin D Binding蛋白抗体[19E8]
参阅全部 Vitamin D Binding protein 一抗 -
描述
小鼠单克隆抗体[19E8] to Vitamin D Binding蛋白 -
宿主
Mouse -
经测试应用
适用于: ELISA, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Vitamin D Binding Protein isolated from human plasma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
19E8 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG2 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23485于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
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别名
- DBP antibody
- DBP/GC antibody
- GC antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (ab23485)IHC image of ab23485 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab23485 尚未被引用在任何文献中。