Anti-Visfatin 抗体 [EPR21980]
Anti-Visfatin antibody [EPR21980]
- RabMAb
- Recombinant
- 20ul selling size
- 了解详情
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(31 Publications)
Anti-Visfatin antibody [EPR21980] (ab236874) is a rabbit monoclonal antibody detecting Visfatin in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
查看别名
PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Visfatin antibody [EPR21980] (AB236874)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID : 26396920) is observed. Counterstained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Visfatin antibody [EPR21980] (AB236874)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in K562 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HCT 116 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human breast cancer (PMID : 21784959) is observed. Counterstained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Unknown
Immunoprecipitation - Anti-Visfatin antibody [EPR21980] (AB236874)
Visfatin was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab236874 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236874 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1 : K562 whole cell lysate 10 μg (input).
Lane 2 : ab236874 IP in K562 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab236874 in K562 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.
All lanes:
Immunoprecipitation - Anti-Visfatin antibody [EPR21980] (ab236874)
Predicted band size: 55 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID : 29104634; PMID : 24287278) is observed. Counterstained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [EPR21980] (AB236874)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in rat liver (PMID : 29104634; PMID : 24603648) is observed. Counterstained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-Visfatin antibody [EPR21980] (AB236874)
Blocking/Diluting buffer and concentration : 5% NFDM/TBST
Exposure times :
Lanes 1-2 : 26 seconds
Lanes 3-5 : 92 seconds
Lane 6 : 3 minutes
Lane 6 was developed using a higher sensitivity ECL substrate.
The molecular weight observed is consistent with what has been described in the literature (PMID : 25368373, PMID : 12782293).
All lanes:
Western blot - Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution
Lane 1:
K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2:
Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg
Lane 3:
HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4:
HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 5:
PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 55 kDa
false
- WB
Supplier Data
Western blot - Anti-Visfatin antibody [EPR21980] (AB236874)
Blocking/Diluting buffer and concentration : 5% NFDM/TBST
Exposure time :
Lanes 1-3 : 40 seconds
Lanes 4-5 : 3 minutes
Lane 6 : 15 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID : 25368373, PMID : 12782293).
All lanes:
Western blot - Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution
Lane 1:
MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2:
HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3:
HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 4:
C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 5:
PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 6:
RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 55 kDa
false
不同偶联物与剂型 (4)
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Anti-Visfatin antibody [EPR21980] - BSA and Azide free (Capture)
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Anti-Visfatin antibody [EPR21980] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Visfatin antibody [EPR21980]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Visfatin antibody [EPR21980]
反应性数据
产品详情
What is this antibody validated in?
Anti-Visfatin antibody [EPR21980] (ab236874) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Visfatin?
Anti-Visfatin [EPR21980] (ab236874) specifically detects a band for Visfatin (UniProt: P43490) at a molecular weight of 55kDa.
Trusted by the scientific community
Anti-Visfatin [EPR21980] (ab236874) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR21980] also available for your convenience: ab236874, Carrier free - ab240589, Carrier free - ab267449, Alexa Fluor® 647 - ab318236, Alexa Fluor® 488 - ab319013
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
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分装信息
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产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (31)
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