重组Anti-Visfatin抗体[BLR058F] (ab272084)
![Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab272084)](https://www.abcam.cn/ps/products/272/ab272084/Images/ab272084-1-ab252558343256RabbitmonoclonalBLR058FtoVisfatinimmunoprecipitationHEK293Thuman.jpg "Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab272084)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR058F] to Visfatin
- Suitable for: IP, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Visfatin抗体[BLR058F]
参阅全部 Visfatin 一抗 -
描述
兔单克隆抗体[BLR058F] to Visfatin -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Visfatin aa 400-450. The exact sequence is proprietary. NP_005737.1
Database link: P43490 -
阳性对照
- IHC-P: Human breast carcinoma and ovarian carcinoma tissue. ICC: NCI-H460. IP: Visfatin IP in HEK-293T whole cell lysate.
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常规说明
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
无载体
是 -
Concentration information loading... -
纯化说明
Purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR058F -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272084于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IP
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
组织特异性
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
通路
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
序列相似性
Belongs to the NAPRTase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10135 Human
- Omim: 608764 Human
- SwissProt: P43490 Human
- Unigene: 489615 Human
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别名
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
图片
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Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab272084)
Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252558 used for IP at 5 µl per reaction. For WB 1/1000 dilution.Lane 1: ab252558 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293Twhole cell lysate.Chemiluminescence detection: 75 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab272084)](https://www.abcam.cn/ps/products/272/ab272084/Images/ab272084-2-ab252558343255RabbitmonoclonalBLR058FtoVisfatinimmunohistochemistryovariancarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab272084)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab272084)](https://www.abcam.cn/ps/products/272/ab272084/Images/ab272084-3-ab252558343254RabbitmonoclonalBLR058FtoVisfatinimmunohistochemistrybreastcarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab272084)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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![Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab272084)](https://www.abcam.cn/ps/products/272/ab272084/Images/ab272084-4-ab252558343253RabbitmonoclonalBLR058FtoVisfatinimmunocytochemistryNCIH460human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab272084)FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
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![Anti-Visfatin antibody [BLR058F] (ab272084)](https://www.abcam.cn/ps/products/272/ab272084/Images/ab272084-5-benefits-of-recombinant-antibodies.png)
Anti-Visfatin antibody [BLR058F] (ab272084)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272084 尚未被引用在任何文献中。