Anti-Vimentin (phospho S82)抗体[EP1071Y] (ab52943)
![Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)](https://www.abcam.cn/ps/products/52/ab52943/Images/ab52943-256530-anti-vimentin-phospho-s82-antibody-ep1071y-dot-blot.jpg "Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)")
Key features and details
- Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
- Suitable for: Dot blot, WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-Vimentin (phospho S82)抗体[EP1071Y]
参阅全部 Vimentin 一抗 -
描述
兔单克隆抗体[EP1071Y] to Vimentin (phospho S82) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysate (AP untreated) + Calyculin A.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1071Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52943于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
1/1000.
|
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| WB |
1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
| 说明 |
|---|
|
Dot blot
1/1000. |
|
WB
1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
靶标
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功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
see all -
形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
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Dot Blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking and dilution buffer: 5% NFDM/TBST
Lane 1 sample: Vimentin (pS83) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide
Exposure time: 3 minutes
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![Western blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)](https://www.abcam.cn/ps/products/52/ab52943/Images/ab52943-65463-anti-vimentin-phospho-s82-antibody-ep1071y-western-blot.gif)
Western blot - Anti-Vimentin (phospho S83) antibody [EP1071Y] (ab52943)All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution
Lane 1 : HeLa cell lysate AP treated
Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab52943 被引用在 4 文献中.
- Liu J et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
- Goto H et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). PubMed: 26795468
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB, ICC/IF ; Human . PubMed: 24073199
- Mähönen AJ et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502
