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RabMAb

Anti-Vimentin (phospho S73)抗体[EP1070Y] (ab52944)

Submit a review Q&A (1)References (4)
Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
  • Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
  • Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)

Key features and details

  • Rabbit monoclonal [EP1070Y] to Vimentin (phospho S73)
  • Suitable for: WB, IP, Dot blot
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Vimentin (phospho S73)抗体[EP1070Y]
    参阅全部 Vimentin 一抗

  • 描述

    兔单克隆抗体[EP1070Y] to Vimentin (phospho S73)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, Dot blotmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa; NIH/3T3; C6 lysates. IP: HeLa cells (untreated and Calyculin A treated).

  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52944于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP
1/20.

For unpurified use at 1:40. 
Dot blot
Use at an assay dependent concentration.
说明
WB
1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP
1/20.

For unpurified use at 1:40. 
Dot blot
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 功能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • 组织特异性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • 疾病相关

    Cataract 30

  • 序列相似性

    Belongs to the intermediate filament family.

  • 结构域

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • 翻译后修饰

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 形式

    Vimentin is found in connective tissue and in the cytoskeleton.

  • 别名

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

图片

  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    All lanes : Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate, untreated
    Lane 2 : C6 (Rat glial tumor glial cell) starved for 24 hours, then treated with 100ng Calyculin A for 60 minutes, whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)

    ab52944 (purified) at 1:20 dilution (2µg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
    Lane 2 (+): ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    For western blotting, VeriBlot for IP Detection Reageant (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    All lanes : Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

    Lane 1 : Untreated with HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved for 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer : 5% NFDM/TBST

  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    All lanes : Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

    Lane 1 : Untreated with NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)

    Primary antibody dilution: 1/1000
    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
    Blocking & dilutiing buffer: 5% NFDM/TBST

    Lane 1 sample: Vimentin (pS72) phospho peptide
    Lane 2 sample: Vimentin non-phospho peptide

    Exposure time: 3 minutes

  • Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
    All lanes : Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/1000000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab52944？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab52944 被引用在 4 文献中.

  • Ohara M  et al. Phosphorylated vimentin as an immunotherapeutic target against metastatic colorectal cancer. Cancer Immunol Immunother 69:989-999 (2020). PubMed: 32086539
  • Liu J  et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
  • Goto H  et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). PubMed: 26795468
  • Mähönen AJ  et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502

客户评价及客户问答

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Question

I want to verify that the recommended dilution for WB on your datasheet is correct: WB: 1/1000000.

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Answer

The lab has confirmed that the recommended dilution for WB is 1:1000000.

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