重组Anti-Vimentin (phospho S38)抗体[EP1069Y] (ab52942)
![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.cn/ps/products/52/ab52942/Images/ab52942-284752-anti-vimentin-phospho-s38-antibody-ep1069y-western-blot.jpg "Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Vimentin (phospho S38)抗体[EP1069Y]
参阅全部 Vimentin 一抗 -
描述
兔单克隆抗体[EP1069Y] to Vimentin (phospho S38) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,IHC-Fr,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
Database link: P08670 -
阳性对照
- WB: HeLa cell lysate treated with Calyculin A.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1069Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
|
| Dot blot |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
|
Dot blot
1/1000. |
应用说明
Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
靶标
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功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
- Omim: 193060 Human
- SwissProt: P08670 Human
- SwissProt: P20152 Mouse
- SwissProt: P31000 Rat
- Unigene: 455493 Human
see all -
形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
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Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution
Lane 1 : HeLa whole cell lysate - untreated
Lane 2 : HeLa whole cell lysate - treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.cn/ps/products/52/ab52942/Images/ab52942-255472-anti-vimentin-phospho-s38-antibody-ep1069y-dot-blot.jpg)
Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.cn/ps/products/52/ab52942/Images/ab52942-65462-anti-vimentin-phospho-s38-antibody-ep1069y-western-blot.gif)
Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate Calyculin A treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa -
![Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)](https://www.abcam.cn/ps/products/52/ab52942/Images/ab52942-6-benefits-of-recombinant-antibodies.png)
Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (8)
ab52942 被引用在 8 文献中.
- Guo Q et al. Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium. Front Bioeng Biotechnol 10:735465 (2022). PubMed: 35547156
- Lone AM et al. Systems approach reveals distinct and shared signaling networks of the four PGE2 receptors in T cells. Sci Signal 14:eabc8579 (2021). PubMed: 34609894
- Liu J et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258
- Chi J et al. Classical intracranial chondrosarcoma: A case report. Oncol Lett 12:4051-4053 (2016). IHC ; Human . PubMed: 27895770
- Kajita M et al. Filamin acts as a key regulator in epithelial defence against transformed cells. Nat Commun 5:4428 (2014). PubMed: 25079702
- Haolong C et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). ICC/IF ; Human . PubMed: 24073199
- Mähönen AJ et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). PubMed: 19903502
- Kim H et al. Filamin A is required for vimentin-mediated cell adhesion and spreading. Am J Physiol Cell Physiol 298:C221-36 (2010). PubMed: 19776392
