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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Vimentin抗体[VI-RE/1] (ab3974)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (4)

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Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
  • Immunocytochemistry - Anti-Vimentin antibody [VI-RE/1] (ab3974)
  • Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
  • Western blot - Anti-Vimentin antibody [VI-RE/1] (ab3974)

Key features and details

  • Mouse monoclonal [VI-RE/1] to Vimentin
  • Suitable for: Flow Cyt (Intra), ICC, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Vimentin抗体[VI-RE/1]
    参阅全部 Vimentin 一抗
  • 描述

    小鼠单克隆抗体[VI-RE/1] to Vimentin
  • 宿主

    Mouse
  • 特异性

    The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
  • 经测试应用

    适用于: Flow Cyt (Intra), ICC, WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rat不与反应: Mouse, Pig
  • 免疫原

    Recombinant full length protein corresponding to Human Vimentin.

  • 阳性对照

    • ICC: HeLa cells. IHC-P: Human bladder and liver tissue. Flow Cyt (Intra): Human ESS-1 and HeLa cells. WB: HeLa whole cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    VI-RE/1
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • APC Anti-Vimentin antibody [VI-RE/1] (ab176512)
    • PE Anti-Vimentin antibody [VI-RE/1] (ab49918)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Related Products

    • Human Vimentin Profiling ELISA Kit (ab173190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3974于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC
Use a concentration of 5 - 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 57 kDa.

Positive control: LEP-19 cell lysate Negative control: 3T3 mouse cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath.

IHC-P (1)
1/400.
说明
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC
Use a concentration of 5 - 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 57 kDa.

Positive control: LEP-19 cell lysate Negative control: 3T3 mouse cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath.

IHC-P
1/400.

靶标

  • 功能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关

    Cataract 30
  • 序列相似性

    Belongs to the intermediate filament family.
  • 结构域

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7431 Human
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P08670 Human
    • SwissProt: P31000 Rat
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 2710 Rat
    • 形式

      Vimentin is found in connective tissue and in the cytoskeleton.
    • 别名

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)

      Flow cytometry (Intracellular) analysis ESS-1 (human endometrial stromal sarcoma cell line) cells labeling Vimentin with ab3974 (GAM-FITC).

      Negative control: human lymphocytes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)

      Immunohistochemistry staining (paraffin sections) of Vimentin in human liver using mouse monoclonal antibody [VI-RE/1] (ab3974, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-RE/1] (ab3974)

      IHC image of Vimentin staining in human bladder formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3974, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemistry - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Immunocytochemistry - Anti-Vimentin antibody [VI-RE/1] (ab3974)

      ICC image of ab3974 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3974, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Flow Cytometry (Intracellular) - Anti-Vimentin antibody [VI-RE/1] (ab3974)

      Flow Cytometry (Intracellular) analysis of HeLa cells labeling Vimentin with ab3974 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3974, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Western blot - Anti-Vimentin antibody [VI-RE/1] (ab3974)
      Anti-Vimentin antibody [VI-RE/1] (ab3974) at 5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Observed band size: 57 kDa why is the actual band size different from the predicted?

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab3974?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3974 被引用在 4 文献中.

    • Li B  et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p. Mol Ther Nucleic Acids 19:814-826 (2020). PubMed: 31958697
    • Chen X  et al. Down-regulation of microRNA-31-5p inhibits proliferation and invasion of osteosarcoma cells through Wnt/ß-catenin signaling pathway by enhancing AXIN1. Exp Mol Pathol 108:32-41 (2019). PubMed: 30844369
    • Hoffmann M  et al. Apelin abrogates the stimulatory effects of 17ß-estradiol and insulin-like growth factor-1 on proliferation of epithelial and granulosa ovarian cancer cell lines via crosstalk between APLNR and ERa/IGF1R. Mol Biol Rep 46:6325-6338 (2019). PubMed: 31538301
    • Kitayama J  et al. CD90+ mesothelial-like cells in peritoneal fluid promote peritoneal metastasis by forming a tumor permissive microenvironment. PLoS One 9:e86516 (2014). Flow Cyt ; Human . PubMed: 24466130

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody [VI-RE/1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human skin (foreskin))
    Permeabilization
    No
    Specification
    Human skin (foreskin)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Unfixed
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [VI-RE/1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human foreskin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 8.3
    Permeabilization
    Yes - 5 minutes w/ PBS-Triton 0.1%
    Specification
    Human foreskin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 02 2020

    Question

    i would like to know if this and the oter antibody as is mention ed on your site ab3974 can be used in elisa, how it is used, i.e which is used as 1st, 2 nd antibody and what has been used for detection, also is there a way i can get a small sample so i can test it for myself before i place an order

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2005

    Answer

    Thank you for your enquiry. Ab7752 and ab3974 can be used together in a sandwich ELISA. As both are unconjugated, you will have to use a secondary antibody for detection, just make sure that the secondary antibody does not cross-react with the coating antibody or the sample. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement.

    Read More

    Abcam Scientific Support

    回复于 Oct 31 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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