AB8979

Anti-Vimentin 抗体 [RV203]

Name: Vimentin抗体[RV203] (ab8979)| Abcam中文官网
Brand: Abcam Limited
SKU: AB8979
Price: 2984 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-Vimentin antibody [RV203]

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(3 Reviews)

(40 Publications)

Mouse Monoclonal Vimentin antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow VIM.

查看别名

Vimentin, VIM

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (AB8979)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (AB8979)

IHC image of ab8979 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8979, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry - Anti-Vimentin antibody [RV203] (AB8979)

Flow Cyt

Unknown

Flow Cytometry - Anti-Vimentin antibody [RV203] (AB8979)

Overlay histogram showing HeLa cells stained with ab8979 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8979, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (AB8979)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (AB8979)

Immunohistochemistry with ab8979 on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (AB8979)

Immunohistochemistry on paraffin section of human colon

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

RV203

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

IHC-Fr, Flow Cyt, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Cow VIM.

P48616

特异性

This antibody reacts exclusively with vimentin which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5 g/mL", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent." } } }

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产品详情

Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VIM

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文献 (40)

Recent publications for all applications. Explore the full list and refine your search

Current therapeutic research, clinical and experimental 102:100765 PubMed39816494

2025

Alpha-Lipoic Acid-Mediated Inhibition of LTB Synthesis Suppresses Epithelial-Mesenchymal Transition, Modulating Functional and Tumorigenic Capacities in Non-Small Cell Lung Cancer A549 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

María José Torres,Juan Carlos Ríos,Alexandra Valle,Sebastián Indo,Kevin Brockway Gv,Fernanda López-Moncada,Mario Faúndez,Enrique A Castellón,Héctor R Contreras

American journal of physiology. Lung cellular and molecular physiology 327:L487-L502 PubMed39104319

2024

Adiponectin suppresses stiffness-dependent, profibrotic activation of lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Nemeth,Wioletta Skronska-Wasek,Sophie Keppler,Annika Schundner,Alexander Groß,Tanja Schoenberger,Karsten Quast,Karim C El Kasmi,Clemens Ruppert,Andreas Günther,Manfred Frick

Nature communications 15:5834 PubMed38992003

2024

A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Gernot Neumayer,Jessica L Torkelson,Shengdi Li,Kelly McCarthy,Hanson H Zhen,Madhuri Vangipuram,Marius M Mader,Gulilat Gebeyehu,Taysir M Jaouni,Joanna Jacków-Malinowska,Avina Rami,Corey Hansen,Zongyou Guo,Sadhana Gaddam,Keri M Tate,Alberto Pappalardo,Lingjie Li,Grace M Chow,Kevin R Roy,Thuylinh Michelle Nguyen,Koji Tanabe,Patrick S McGrath,Amber Cramer,Anna Bruckner,Ganna Bilousova,Dennis Roop,Jean Y Tang,Angela Christiano,Lars M Steinmetz,Marius Wernig,Anthony E Oro

Journal of gastrointestinal oncology 14:2178-2191 PubMed37969822

2023

HMGB2 upregulation promotes the progression of hepatocellular carcinoma cells through the activation of ZEB1/vimentin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Lu,Teng Zhao,Lixue Yang,Yang Liu,Xiang Ruan,Longjiu Cui,Yongjie Zhang

JACC. Basic to translational science 8:862-880 PubMed37547071

2023

Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohisa Sakaue,Tadaaki Koyama,Yoshitsugu Nakamura,Keitaro Okamoto,Takayuki Kawashima,Tadashi Umeno,Yasuhide Nakayama,Shinji Miyamoto,Fumiaki Shikata,Mika Hamaguchi,Jun Aono,Mie Kurata,Kenji Namiguchi,Shunji Uchita,Junya Masumoto,Osamu Yamaguchi,Shigeki Higashiyama,Hironori Izutani

Biomedicines 11: PubMed37509721

2023

Tumor-Associated Macrophages Affect the Tumor Microenvironment and Radioresistance via the Upregulation of CXCL6/CXCR2 in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Lun Lee,Yi-Chieh Tsai,Narpati Wesa Pikatan,Chi-Tai Yeh,Vijesh Kumar Yadav,Ming-Yao Chen,Jo-Ting Tsai

Chinese medical journal 136:2351-2361 PubMed37036437

2023

Long non-coding RNA colon cancer-associated transcript 1-Vimentin axis promoting the migration and invasion of HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangfu Li,Jiangbei Yuan,Qingen Da,Zilong Yan,Jianhua Qu,Dan Li,Xu Liu,Qimin Zhan,Jikui Liu

Journal of immunology research 2022:4996980 PubMed35874898

2022

hsa_circ_0000518 Facilitates Non-Small-Cell Lung Cancer Progression via Moderating miR-330-3p and Positively Regulating SLC1A5.

Applications

Unspecified application

Species

Unspecified reactive species

Huilai Lv,Zhihua Shi,Aixia Sui,Yan Zhang,Liangbiao Peng,Mingbo Wang,Fan Zhang

Molecular cell 82:2858-2870.e8 PubMed35732190

2022

Supramolecular assembly of GSK3α as a cellular response to amino acid starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Hinze,Sabine Schreek,Andre Zeug,Nurul Khalida Ibrahim,Beate Fehlhaber,Lorent Loxha,Buesra Cinar,Evgeni Ponimaskin,James Degar,Connor McGuckin,Gabriela Chiosis,Cornelia Eckert,Gunnar Cario,Beat Bornhauser,Jean-Pierre Bourquin,Martin Stanulla,Alejandro Gutierrez

International journal of molecular sciences 23: PubMed35743073

2022

Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Bond,Vito Bruno,Jason Johnson,Sarah George,Raimondo Ascione

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin 抗体 [RV203]

Anti-Vimentin antibody [RV203]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (AB8979)

Flow Cytometry - Anti-Vimentin antibody [RV203] (AB8979)

Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (AB8979)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (AB8979)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性