Anti-Vimentin抗体(ab188499)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Vimentin抗体
参阅全部 Vimentin 一抗 -
描述
兔多克隆抗体to Vimentin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Mammals, Cynomolgus monkey -
免疫原
Recombinant full length protein corresponding to Human Vimentin aa 1 to the C-terminus. (Purified from E. coli).
Database link: P08670 -
阳性对照
- WB: HeLa cell extract; HeLa, SH-SY5Y, HEK-293 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.07% Sodium azide -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/5000.
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WB |
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
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说明 |
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ICC/IF
1/5000. |
WB
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa). |
靶标
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功能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
组织特异性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
疾病相关
Cataract 30 -
序列相似性
Belongs to the intermediate filament family. -
结构域
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻译后修饰
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 493952 Chimpanzee
- Entrez Gene: 101864871 Cynomolgus monkey
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Omim: 193060 Human
- SwissProt: Q5R1W8 Chimpanzee
- SwissProt: Q4R4X4 Cynomolgus monkey
- SwissProt: P08670 Human
see all -
形式
Vimentin is found in connective tissue and in the cytoskeleton. -
别名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
图片
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.
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All lanes : Anti-Vimentin antibody (ab188499) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Predicted band size: 53 kDa -
Anti-Vimentin antibody (ab188499) at 1/10000 dilution + HeLa cell extract
Developed using the ECL technique.
Predicted band size: 53 kDa -
Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab188499 被引用在 4 文献中.
- Cortese M et al. Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies. Cell Host Microbe 28:853-866.e5 (2020). PubMed: 33245857
- Sacco A et al. Platelet-Specific Deletion of Cyclooxygenase-1 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice. J Pharmacol Exp Ther 370:416-426 (2019). PubMed: 31248980
- Wang B et al. miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/ß-catenin pathway in non-small cell lung cancer. Mol Med Rep 18:2753-2761 (2018). PubMed: 30015869
- Cao Z et al. [ARTICLE WITHDRAWN] MicroRNA-539 Inhibits the Epithelial-Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4. Oncol Res 26:529-536 (2018). PubMed: 28653599