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AB317112

Anti-Villin antibody [HL1830]

Anti-Villin antibody [HL1830]

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Rabbit Monoclonal Villin antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human VIL1.

查看别名

VIL, VIL1, Villin-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [HL1830] (AB317112)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody [HL1830] (AB317112)

ab317112 detects Villin protein at cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded mouse duodenum. Villin stained by ab317112 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317112 diluted at 1 : 4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/4000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE and the membranes were blotted with ab317112 diluted at 1 : 4000 and competitor's antibody diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/4000 dilution

All lanes:

HepG2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

false

Western blot - Anti-Villin antibody [HL1830] (AB317112)
  • WB

Supplier Data

Western blot - Anti-Villin antibody [HL1830] (AB317112)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317112 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Villin antibody [HL1830] (ab317112) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

DDDDK-tagged Villin transfected (+) 293T whole cell extracts

Lane 3:

DDDDK-tagged Advillin transfected (+) 293T whole cell extracts

Lane 4:

DDDDK-tagged Gelsolin transfected (+) 293T whole cell extracts

Lane 5:

DDDDK-tagged Villin like transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1830

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human VIL1.

P09327

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1
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Abcam Product Promise

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