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AB109516

重组Anti-Villin抗体[EPR3490]

Anti-Villin antibody [EPR3490]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal Villin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

查看别名

VIL, VIL1, Villin-1

2 Images
Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Villin antibody [EPR3490] (AB109516)

Intracellular flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-Villin antibody [EPR3490] (AB109516)
  • WB

Unknown

Western blot - Anti-Villin antibody [EPR3490] (AB109516)

All lanes:

Western blot - Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Caco-2 lysate at 10 µg

Predicted band size: 93 kDa

Observed band size: 95 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3490

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
See full target information VIL1

文献 (7)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 104:105181 PubMed38838469

2024

Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeru Fujita,Arnon Plianchaisuk,Sayaka Deguchi,Hayato Ito,Naganori Nao,Lei Wang,Hesham Nasser,Tomokazu Tamura,Izumi Kimura,Yukie Kashima,Rigel Suzuki,Saori Suzuki,Izumi Kida,Masumi Tsuda,Yoshitaka Oda,Rina Hashimoto,Yukio Watanabe,Keiya Uriu,Daichi Yamasoba,Ziyi Guo,Alfredo A Hinay,Yusuke Kosugi,Luo Chen,Lin Pan,Yu Kaku,Hin Chu,Flora Donati,Sarah Temmam,Marc Eloit,Yuki Yamamoto,Tetsuharu Nagamoto,Hiroyuki Asakura,Mami Nagashima,Kenji Sadamasu,Kazuhisa Yoshimura,Yutaka Suzuki,Jumpei Ito,Terumasa Ikeda,Shinya Tanaka,Keita Matsuno,Takasuke Fukuhara,Kazuo Takayama,Kei Sato

Biotechnology journal 19:e2300390 PubMed38375564

2024

A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiong Guo,Yingying Xie,Jianhua Qin

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Molecular therapy. Methods & clinical development 17:49-57 PubMed31890740

2020

Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Kanae Kawai,Ryosuke Negoro,Moe Ichikawa,Tomoki Yamashita,Sayaka Deguchi,Kazuo Harada,Kazumasa Hirata,Kazuo Takayama,Hiroyuki Mizuguchi

Scientific reports 8:16393 PubMed30401824

2018

Aggregated SOD1 causes selective death of cultured human motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Benkler,Alison L O'Neil,Susannah Slepian,Fang Qian,Paul H Weinreb,Lee L Rubin

Biochemical and biophysical research communications 472:631-6 PubMed26966071

2016

Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells.

Applications

Flow Cyt

Species

Human

Ryosuke Negoro,Kazuo Takayama,Yasuhito Nagamoto,Fuminori Sakurai,Masashi Tachibana,Hiroyuki Mizuguchi

Scientific reports 5:16479 PubMed26559489

2015

Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine.

Applications

Flow Cyt

Species

Human

Tatsuya Ozawa,Kazuo Takayama,Ryota Okamoto,Ryosuke Negoro,Fuminori Sakurai,Masashi Tachibana,Kenji Kawabata,Hiroyuki Mizuguchi
View all publications

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