重组Anti-VILIP1抗体[EPR9832] (ab180141)
![Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)](https://www.abcam.cn/ps/products/180/ab180141/Images/ab180141-210021-anti-vilip1-antibody-epr9832-western-blot.jpg "Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9832] to VILIP1
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-VILIP1抗体[EPR9832]
参阅全部 VILIP1 一抗 -
描述
兔单克隆抗体[EPR9832] to VILIP1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human VILIP1 aa 1-100. The exact sequence is proprietary.
Database link: P62760 -
阳性对照
- Human cerebellum, Mouse brain and rat brain lysate, Human brain tissue, SH-SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9832 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180141于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 22 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 22 kDa). |
靶标
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功能
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. -
组织特异性
Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level). -
序列相似性
Belongs to the recoverin family.
Contains 4 EF-hand domains. - Information by UniProt
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数据库链接
- Entrez Gene: 7447 Human
- Entrez Gene: 26950 Mouse
- Entrez Gene: 24877 Rat
- Omim: 600817 Human
- SwissProt: P62760 Human
- SwissProt: P62761 Mouse
- SwissProt: P62762 Rat
- Unigene: 444212 Human
see all -
别名
- 21 kDa CABP antibody
- Hippocalcin like protein 3 antibody
- Hippocalcin-like protein 3 antibody
see all
图片
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Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)All lanes : Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9832] (ab180141)](https://www.abcam.cn/ps/products/180/ab180141/Images/ab180141-210022-anti-vilip1-antibody-epr9832-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VILIP1 antibody [EPR9832] (ab180141)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab180141 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-VILIP1 antibody [EPR9832] (ab180141)](https://www.abcam.cn/ps/products/180/ab180141/Images/ab180141-210023-anti-vilip1-antibody-epr9832-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-VILIP1 antibody [EPR9832] (ab180141)Intracellular flow cytometric analysis of 2% paraformaldehyde fixedSH-SY5Y cells labeling VILIP1 with ab180141 at 1/50. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.
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![Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)](https://www.abcam.cn/ps/products/180/ab180141/Images/ab180141-210020-anti-vilip1-antibody-epr9832-western-blot.jpg)
Western blot - Anti-VILIP1 antibody [EPR9832] (ab180141)Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution + Human cerebellum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST
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![Anti-VILIP1 antibody [EPR9832] (ab180141)](https://www.abcam.cn/ps/products/180/ab180141/Images/ab180141-6-benefits-of-recombinant-antibodies.png)
Anti-VILIP1 antibody [EPR9832] (ab180141)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab180141 被引用在 1 文献中.
- Gu J et al. Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p. Exp Ther Med 26:503 (2023). PubMed: 37822583