Anti-VGLUT3抗体[N34/34] (ab134310)
Key features and details
- Mouse monoclonal [N34/34] to VGLUT3
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-VGLUT3抗体[N34/34]
参阅全部 VGLUT3 一抗 -
描述
小鼠单克隆抗体[N34/34] to VGLUT3 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment (His-tag) within Rat VGLUT3 aa 500 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q7TSF2 -
阳性对照
- ICC/IF: SK-N-BE cells.
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常规说明
The clone number has been updated from S34-34 to N34/34, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N34/34 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134310于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
IHC-P | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/100.
|
说明 |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/100. |
靶标
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功能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
组织特异性
Expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord and thalamus. -
疾病相关
Defects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25) [MIM:605583]. DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age-dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged. -
序列相似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 246213 Human
- Entrez Gene: 216227 Mouse
- Entrez Gene: 266767 Rat
- Omim: 607557 Human
- SwissProt: Q8NDX2 Human
- SwissProt: Q8BFU8 Mouse
- SwissProt: Q7TSF2 Rat
- Unigene: 116871 Human
see all -
别名
- deafness autosomal dominant 25 antibody
- DFNA 25 antibody
- DFNA25 antibody
see all
图片
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Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling VGLUT3 using ab134310 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134310 被引用在 1 文献中.
- Zhang XL et al. Reorganization of postmitotic neuronal chromatin accessibility for maturation of serotonergic identity. Elife 11:N/A (2022). PubMed: 35471146