Anti-VGluT1抗体(ab77822)
Key features and details
- Rabbit polyclonal to VGluT1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VGluT1抗体
参阅全部 VGluT1 一抗 -
描述
兔多克隆抗体to VGluT1 -
宿主
Rabbit -
特异性
Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Cow, Pigeon, Human, Zebrafish
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免疫原
Synthetic peptide corresponding to Rat VGluT1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93078) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa). |
靶标
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功能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
组织特异性
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. -
序列相似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 518849 Cow
- Entrez Gene: 57030 Human
- Entrez Gene: 72961 Mouse
- Entrez Gene: 116638 Rat
- Omim: 605208 Human
- SwissProt: A4FV52 Cow
- SwissProt: Q9P2U7 Human
- SwissProt: Q3TXX4 Mouse
see all -
别名
- BNPI antibody
- Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
- Brain specific Na dependent inorganic phosphate cotransporter antibody
see all
图片
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Western blot - Anti-VGluT1 antibody (ab77822)All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Cerebellum Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab77822 被引用在 23 文献中.
- Aloi MS et al. Microglia specific deletion of miR-155 in Alzheimer's disease mouse models reduces amyloid-β pathology but causes hyperexcitability and seizures. J Neuroinflammation 20:60 (2023). PubMed: 36879321
- Albrecht NE et al. Rapid 3D-STORM imaging of diverse molecular targets in tissue. Cell Rep Methods 2:100253 (2022). PubMed: 35880013
- Peters JJ et al. Molecular Characterization of AMPA-Receptor-Containing Vesicles. Front Mol Neurosci 14:754631 (2021). PubMed: 34720876
- Garita-Hernandez M et al. Control of Microbial Opsin Expression in Stem Cell Derived Cones for Improved Outcomes in Cell Therapy. Front Cell Neurosci 15:648210 (2021). PubMed: 33815066
- DeMarco E et al. Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis. Front Neuroanat 15:636683 (2021). PubMed: 33613200