重组Anti-Versican抗体[EPR12277] (ab177480)
![Western blot - Anti-Versican antibody [EPR12277] (ab177480)](https://www.abcam.cn/ps/products/177/ab177480/Images/ab177480-316243-anti-versican-antibody-epr12277-western-blot.jpg "Western blot - Anti-Versican antibody [EPR12277] (ab177480)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12277] to Versican
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Versican抗体[EPR12277]
参阅全部 Versican 一抗 -
描述
兔单克隆抗体[EPR12277] to Versican -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Versican aa 3200-3300 (Cysteine residue). The exact sequence is proprietary.
Database link: P13611 -
阳性对照
- WB: Fetal brain, fetal heart, rat and mouse hippocampal, mouse heart lysates;
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12277 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177480于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 373 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 373 kDa. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. -
组织特异性
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. -
疾病相关
Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. -
序列相似性
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
发展阶段
Disappears after the cartilage development. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1462 Human
- Entrez Gene: 13003 Mouse
- Entrez Gene: 114122 Rat
- Omim: 118661 Human
- SwissProt: P13611 Human
- SwissProt: Q62059 Mouse
- SwissProt: Q9ERB4 Rat
- Unigene: 643801 Human
see all -
别名
- Chondroitin sulfate proteoglycan 2 antibody
- Chondroitin sulfate proteoglycan core protein 2 antibody
- Chondroitin sulfate proteoglycan core protein, cartilage antibody
see all
图片
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Western blot - Anti-Versican antibody [EPR12277] (ab177480)All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 0.1 µg/ml (purified)
Lane 1 : Human hippocampus lysate
Lane 2 : Mouse hippocampus lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat hippocampus lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 373 kDaBlocking and diluting buffer: 5% NFDM/TBST
This antibody detects multi-bands due to the splicing variants and proteolytic fragments (PMID: 27683612, 24401530, 29222454, 19662655) -
![Western blot - Anti-Versican antibody [EPR12277] (ab177480)](https://www.abcam.cn/ps/products/177/ab177480/Images/ab177480-205404-anti-versican-antibody-epr12277-western-blot.jpg)
Western blot - Anti-Versican antibody [EPR12277] (ab177480)All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilution (Unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 373 kDa -
![Anti-Versican antibody [EPR12277] (ab177480)](https://www.abcam.cn/ps/products/177/ab177480/Images/ab177480-8-benefits-of-recombinant-antibodies.png)
Anti-Versican antibody [EPR12277] (ab177480)
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab177480 被引用在 24 文献中.
- Kratochvil MJ et al. Biochemical, biophysical, and immunological characterization of respiratory secretions in severe SARS-CoV-2 infections. JCI Insight 7:N/A (2022). PubMed: 35730564
- Yao Q et al. Comparative proteomic and clinicopathological analysis of breast adenoid cystic carcinoma and basal-like triple-negative breast cancer. Front Med (Lausanne) 9:943887 (2022). PubMed: 35966872
- Song J et al. Versican enrichment predicts poor prognosis and response to adjuvant therapy and immunotherapy in gastric cancer. Front Immunol 13:960570 (2022). PubMed: 36203562
- Egerbacher M et al. Stress-deprivation induces an up-regulation of versican and connexin-43 mRNA and protein synthesis and increased ADAMTS-1 production in tendon cells in situ. Connect Tissue Res N/A:1-10 (2021). PubMed: 33467936
- Mutoji KN et al. Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34071909