Anti-VEPH1抗体(ab121875)
Key features and details
- Rabbit polyclonal to VEPH1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VEPH1抗体
参阅全部 VEPH1 一抗 -
描述
兔多克隆抗体to VEPH1 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human VEPH1 aa 1-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14D04 -
阳性对照
- IHC-P: Human lung, kidney, adrenal gland, fallopian tube, and endometrium tissues; ICC: Human cell line A-431.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121875于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the MELT/VEPH family.
Contains 1 PH domain. -
结构域
The PH domain is required for membrane targeting. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 79674 Human
- Omim: 609594 Human
- SwissProt: Q14D04 Human
- Unigene: 658046 Human
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别名
- 2810471M23Rik antibody
- MELT antibody
- MELT_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEPH1 antibody (ab121875)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling VEPH1 with ab121875 at 1/20 dilution. Cytoplasmic staining observed in macrophages and alveolar cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEPH1 antibody (ab121875)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VEPH1 with ab121875 at 1/20 dilution. Membranous staining observed in tubule cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEPH1 antibody (ab121875)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adrenal gland tissue labelling VEPH1 with ab121875 at 1/20 dilution. Membranous and cytoplasmic staining observed in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEPH1 antibody (ab121875)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling VEPH1 with ab121875 at 1/20 dilution. Membranous staining observed in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEPH1 antibody (ab121875)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling VEPH1 with ab121875 at 1/20 dilution. Membranous staining observed in tubule cells.
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Immunocytochemistry - Anti-VEPH1 antibody (ab121875)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cell line A-431 labelling VEPH1 with ab121875 at 2 µg/mL or dilution
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121875 被引用在 2 文献中.
- Fan L et al. MicroRNA miR-23b-3p promotes osteosarcoma by targeting ventricular zone expressed PH domain-containing 1 (VEPH1)/phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. Bioengineered 12:12568-12582 (2021). PubMed: 34903122
- Feng H et al. Overexpressed VEPH1 inhibits epithelial-mesenchymal transition, invasion, and migration of human cutaneous melanoma cells through inactivating the TGF-ß signaling pathway. Cell Cycle 18:2860-2875 (2019). PubMed: 31599708