Anti-VEGFA抗体(ab46154)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-VEGFA抗体
参阅全部 VEGFA 一抗 -
描述
兔多克隆抗体to VEGFA -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab46154 are tested in WB. Previous batches were additionally validated in ELISA. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab214424.
-
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
-
阳性对照
- ab46154 gave a positive result in the following whole cell lysates: Recombinant Human VEGFA protein Recombinant Mouse VEGFA protein HCT116 whole cell lysate
-
常规说明
New batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab52917 as an alternative for ICC/IF and IHC-P.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Control Peptide
-
Immunizing Peptide (Blocking)
-
Immunohistochemistry kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46154于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (16) |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 27 kDa).Can be blocked with Human VEGFA peptide (ab46160).
We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
ELISA |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 27 kDa).Can be blocked with Human VEGFA peptide (ab46160). We recommend Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody. |
ELISA
Use at an assay dependent concentration. |
靶标
-
功能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
组织特异性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
疾病相关
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
细胞定位
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
-
数据库链接
- Entrez Gene: 7422 Human
- Entrez Gene: 22339 Mouse
- Entrez Gene: 83785 Rat
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q96KJ0 Human
- SwissProt: Q00731 Mouse
- SwissProt: P16612 Rat
see all -
别名
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
图片
-
Anti-VEGFA antibody (ab46154) at 1 µg/ml + HCT116 whole cell lysate at 20 µg
Secondary
Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
-
Ab46154 was tested using indirect ELISA. The wells were coated with peptide (1 µg x mL-1 at 100 µL per well) overnight at 4°C, followed by a 1% fat-free milk blocking step for 1 hour at room temperature. The primary antibody (ab46154) was added at a range of dilutions (50 µL per well) for 1 hour at room temperature. Ab97080 (Goat Anti-Rabbit IgG H&L-HRP) was used as a secondary antibody at 1:50.000 dilution for 1 hour at room temperature and signal was developed with TMB substrate.
-
All lanes : Anti-VEGFA antibody (ab46154) at 1 µg/ml
Lane 1 : Recombinant Human VEGFA protein (His tag) (ab204773)
Lane 2 :Recombinant mouse VEGFA protein (Active) (ab185265)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
-
SDS download
-
Datasheet download
文献 (714)
ab46154 被引用在 714 文献中.
- Albalawi MA et al. The medicinal activity of lyophilized aqueous seed extract of Lepidium sativum L. in an androgenic alopecia model. Sci Rep 13:7676 (2023). PubMed: 37169776
- Bychkov I et al. Regulation of VEGFR2 and AKT Signaling by Musashi-2 in Lung Cancer. Cancers (Basel) 15:N/A (2023). PubMed: 37173995
- Jia J et al. LncRNA DANCR Enhances Angiogenesis to Promote Melanoma Progression Via Sponging miR-5194. J Cancer 14:1161-1173 (2023). PubMed: 37215458
- Kim J et al. Investigation of artificial intelligence integrated fluorescence endoscopy image analysis with indocyanine green for interpretation of precancerous lesions in colon cancer. PLoS One 18:e0286189 (2023). PubMed: 37228164
- Cao X et al. Efficient ocular delivery of siRNA via pH-sensitive vehicles for corneal neovascularization inhibition. Int J Pharm X 5:100183 (2023). PubMed: 37234133