Anti-VEGFA抗体(ab183100)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VEGFA抗体
参阅全部 VEGFA 一抗 -
描述
兔多克隆抗体to VEGFA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human VEGFA aa 130-143. The exact sequence is proprietary.
Database link: P15692 -
阳性对照
- IHC-P: Human liver cancer tissue. WB: Hela, Jurkat, HEK-293T, HT29, MDA-MB-231, A549, SK-OV-3 and 231 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
without Mg2+ and Ca2+) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183100于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/450 - 1/2000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/25 - 1/100.
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说明 |
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WB
1/450 - 1/2000. Predicted molecular weight: 27 kDa. |
IHC-P
1/25 - 1/100. |
靶标
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功能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
组织特异性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
疾病相关
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
细胞定位
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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数据库链接
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- SwissProt: Q96KJ0 Human
- Unigene: 73793 Human
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别名
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
see all
图片
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Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling VEGFA with ab183100 at 1/25 dilution. Image on the right is treated with the synthetic peptide.
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All lanes : Anti-VEGFA antibody (ab183100) at 1/450 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : 231 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 27 kDa
Exposure time: 2 minutes -
All lanes : Anti-VEGFA antibody (ab183100) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : HT29 cell lysate
Lane 5 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 6 : A549 (human lung carcinoma cell line) cell lysate
Lane 7 : SK-OV-3 (human ovarian cancer cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/100 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 2 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab183100 被引用在 22 文献中.
- Zhuang X et al. Ocular Manifestations in a Chinese Pedigree of Familial Amyloidotic Polyneuropathy Carrying the Transthyretin Mutation c.401A>G (p.Tyr134Cys). Genes (Basel) 13:N/A (2022). PubMed: 35627273
- Kim YJ et al. Area light source-triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells. Bioeng Transl Med 7:e10255 (2022). PubMed: 35079630
- Tian X et al. miR-144 delivered by nasopharyngeal carcinoma-derived EVs stimulates angiogenesis through the FBXW7/HIF-1α/VEGF-A axis. Mol Ther Nucleic Acids 24:1000-1011 (2021). PubMed: 34094717
- Tekiki N et al. Dynamic contrast-enhanced MRI as a predictor of programmed death ligand-1 expression in patients with oral squamous cell carcinoma. Oncol Lett 22:778 (2021). PubMed: 34594419
- Jin X et al. An Adipose-Derived Injectable Sustained-Release Collagen Scaffold of Adipokines Prepared Through a Fast Mechanical Processing Technique for Preventing Skin Photoaging in Mice. Front Cell Dev Biol 9:722427 (2021). PubMed: 34631708