重组Anti-VEGF Receptor 2抗体[SP123] (ab115805)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)](https://www.abcam.cn/ps/products/115/ab115805/Images/ab115805-4-Anti-VEGF-Receptor-2-antibody-SP123-for-Immunohistochemistry-melanoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP123] to VEGF Receptor 2
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-VEGF Receptor 2抗体[SP123]
参阅全部 VEGF Receptor 2 一抗 -
描述
兔单克隆抗体[SP123] to VEGF Receptor 2 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human placenta, and Human glioma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP123 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-VEGF Receptor 2 antibody [SP123] - BSA and Azide free (ab243912)
- Alexa Fluor® 488 Anti-VEGF Receptor 2 antibody [SP123] (ab320450)
- Alexa Fluor® 647 Anti-VEGF Receptor 2 antibody [SP123] (ab320451)
- Alexa Fluor® 555 Anti-VEGF Receptor 2 antibody [SP123] (ab320452)
- Alexa Fluor® 594 Anti-VEGF Receptor 2 antibody [SP123] (ab320453)
- PE Anti-VEGF Receptor 2 antibody [SP123] (ab320454)
- APC Anti-VEGF Receptor 2 antibody [SP123] (ab320455)
- Alexa Fluor® 750 Anti-VEGF Receptor 2 antibody [SP123] (ab320970)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115805于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
疾病相关
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3791 Human
- Entrez Gene: 16542 Mouse
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: P35918 Mouse
- Unigene: 479756 Human
- Unigene: 285 Mouse
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别名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)This image is courtesy of an Abreview submitted by Daniel KirschImmunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed, 1% TritonX-100 in PBS permeabilized human Metastatic melanoma tissue. Stained with ab115805 at 1/200 dilution. Secondary antibody used was HRP Donkey Jackson Immuno Anti-rabbit at 1/500 dilution. Blocking was done with 3% donkey serum for 30 minutes at 25°C. The sample was incubated with the primary antibody and 1% donkey serum for 1 hour at 25°C.
Antigen retrieval method was heat mediated Tris-EDTA -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)](https://www.abcam.cn/ps/products/115/ab115805/Images/ab115805-2-AntiVEGF-Receptor-2-antibody-SP123-IHC-Human-glioma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling VEGF Receptor 2 with ab115805 at 1/100 dilution (1.72 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)](https://www.abcam.cn/ps/products/115/ab115805/Images/ab115805-127949-anti-vegf-receptor-2-antibody-sp123-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [SP123] (ab115805)Staining of Human VEGF Receptor 2 in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115805 at a dilution of 1/100. -
![Anti-VEGF Receptor 2 antibody [SP123] (ab115805)](https://www.abcam.cn/ps/products/115/ab115805/Images/ab115805-3-benefits-of-recombinant-antibodies.png)
Anti-VEGF Receptor 2 antibody [SP123] (ab115805)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab115805 被引用在 7 文献中.
- Yoysungnoen B et al. Short term effect of tetrahydrocurcumin on adipose angiogenesis in very high-fat diet-induced obesity mouse model. Front Nutr 10:1221935 (2023). PubMed: 37876615
- Duan J et al. Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regeneration. Regen Biomater 9:rbac033 (2022). PubMed: 35719204
- Wang S et al. Network Pharmacological Analysis and Experimental Validation of the Mechanisms of Action of Si-Ni-San Against Liver Fibrosis. Front Pharmacol 12:656115 (2021). PubMed: 34276360
- Aditianingsih D et al. Effects of low versus standard pressure pneumoperitoneum on renal syndecan-1 shedding and VEGF receptor-2 expression in living-donor nephrectomy: a randomized controlled study. BMC Anesthesiol 20:37 (2020). PubMed: 32019488
- Chaudhary A et al. Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis. J Tradit Complement Med 10:529-543 (2020). PubMed: 33134129
- Anspach L et al. Function and mutual interaction of BiP-, PERK-, and IRE1a-dependent signalling pathways in vascular tumours. J Pathol 251:123-134 (2020). PubMed: 32166747
- Jayasinghe C et al. The relevance of cell type- and tumor zone-specific VEGFR-2 activation in locally advanced colon cancer. J Exp Clin Cancer Res 34:42 (2015). PubMed: 25967108