重组Anti-VEGF Receptor 2抗体[EPRER16Y] (ab134191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRER16Y] to VEGF Receptor 2
- Suitable for: ELISA, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-VEGF Receptor 2抗体[EPRER16Y]
参阅全部 VEGF Receptor 2 一抗 -
描述
兔单克隆抗体[EPRER16Y] to VEGF Receptor 2 -
宿主
Rabbit -
特异性
It is predicted that this antibody does not cross react with other members of the VEGF receptor family.
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经测试应用
适用于: ELISA, WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human VEGF Receptor 2 aa 1-800. The exact sequence is proprietary.
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阳性对照
- WB: Human placenta lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPRER16Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa).
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IP |
1/20.
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa). |
IP
1/20. |
靶标
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功能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
疾病相关
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
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别名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
图片
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Anti-VEGF Receptor 2 antibody [EPRER16Y] (ab134191) at 1/500 dilution + Human placenta lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 151 kDa
Observed band size: 210,230 kDa why is the actual band size different from the predicted? -
ELISA analysis of Hu VEGFR2 recombinant protein at 1000 ng/mL with ab134191. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab134191 被引用在 23 文献中.
- Peng Z et al. CCL2 promotes proliferation, migration and angiogenesis through the MAPK/ERK1/2/MMP9, PI3K/AKT, Wnt/β‑catenin signaling pathways in HUVECs. Exp Ther Med 25:77 (2023). PubMed: 36684650
- Sun X & Liu B Donepezil ameliorates oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cell dysfunction via the SIRT1/FOXO3a/NF-κB pathways. Bioengineered 13:7760-7770 (2022). PubMed: 35286233
- Luo Z et al. LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Exp Ther Med 24:622 (2022). PubMed: 36160904
- Wu Q et al. KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1. Exp Ther Med 24:683 (2022). PubMed: 36277155
- Yan J et al. HMGA2 promotes the migration and invasion of gallbladder cancer cells and HMGA2 knockdown inhibits angiogenesis via targeting VEGFA. Mol Med Rep 25:N/A (2022). PubMed: 34913073