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AB2349

Anti-VEGF Receptor 2 抗体

Anti-VEGF Receptor 2 antibody

4

(17 Reviews)

|

(139 Publications)

Anti-VEGF Receptor 2 antibody (ab2349) is a rabbit polyclonal antibody detecting VEGF Receptor 2 in Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Dog, Human, Mouse, Rat.

- Over 140 publications
- Trusted since 2002

查看别名

CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1I. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0/.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal breast tissue labeling VEGF Receptor 2 with ab2349 at 1/200 dilution. Sections were lightly counterstained with hematoxylin.

Image from Arias-Pulido, Hugo et al., BMC Cancer 12 (2012): 298. PMC. Web. 25 Jan. 2017. doi: 10.1186/1471-2407-12-298. Fig 1H. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)

Formalin fixed, paraffin-embedded human angiosarcoma tissue stained for VEGF Receptor 2 using ab2349 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)
  • IHC-P

AbReview19275****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody (AB2349)

ab2349 staining the VEGF Receptor 2 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS + 2% blocking serum) for 16 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Manoj Kumar Valluru

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Dog, Mouse, Cow, Rat

应用

IHC-P, IP, ICC/IF, Flow Cyt, IHC-Fr

applications

免疫原

Synthetic Peptide within Mouse Kdr. The exact immunogen used to generate this antibody is proprietary information.

P35918

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>See Abreview.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>See Abreview.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>See Abreview.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>See Abreview.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>See Abreview.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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您是否考虑以下替代产品？

AB134191

Anti-VEGF Receptor 2 antibody [EPRER16Y]

0
0 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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产品详情

What is this antibody validated in?
Anti-VEGF Receptor 2 antibody (ab2349) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Dog, Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-VEGF Receptor 2 (ab2349) was first used in a scientific publication in 2002 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-VEGF Receptor 2 (ab2349) has over 15 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
Biological function summary

VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.

Pathways

VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.

VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
See full target information KDR
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文献 (139)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 29:155 PubMed37940881

2023

Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Hu,Changwei Lin,Kai Gao,Miao Chen,Fei Long,Buning Tian

Nanoscale 15:17173-17183 PubMed37853851

2023

Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Polo-Montalvo,Mónica Cicuéndez,Laura Casarrubios,Nathalie Barroca,Daniela da Silva,María José Feito,Rosalía Diez-Orejas,María Concepción Serrano,Paula A A P Marques,María Teresa Portolés

Nature communications 14:4893 PubMed37580352

2023

Camrelizumab combined with apatinib in patients with first-line platinum-resistant or PD-1 inhibitor resistant recurrent/metastatic nasopharyngeal carcinoma: a single-arm, phase 2 trial.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yuan,Guo-Dong Jia,Xiao-Fei Lv,Si-Yi Xie,Shan-Shan Guo,Da-Feng Lin,Li-Ting Liu,Dong-Hua Luo,Yi-Fu Li,Shen-Wen Deng,Ling Guo,Mu-Sheng Zeng,Xiu-Yu Cai,Sai-Lan Liu,Xue-Song Sun,Xiao-Yun Li,Su-Chen Li,Qiu-Yan Chen,Lin-Quan Tang,Hai-Qiang Mai

Bioengineering & translational medicine 8:e10562 PubMed37693053

2023

Dual gene-activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Weng,Min Yang,Wei Zhang,Ronghua Jin,Sizhan Xia,Manjia Zhang,Pan Wu,Xiaojie He,Chunmao Han,Xiong Zhao,Xingang Wang

Investigational new drugs 41:226-239 PubMed37004643

2023

Development of a MET-targeted single-chain antibody fragment as an anti-oncogene targeted therapy for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rana Vafaei,Zohreh Khaki,Malihe Salehi,Neda Jalili,Mohammad Reza Esmailinejad,Ahad Muhammadnajad,Seyed Mahdi Nassiri,Alireza Vajhi,Shima Moradi Kalbolandi,Roya Mirzaei,Leila Farahmand

The Tohoku journal of experimental medicine 260:63-73 PubMed36823182

2023

Rhamnazin Enhanced Anti-Tumor Efficacy of Anti-PD-1 Therapy for Lung Cancer in Mice through Inhibition of PD-L1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Shi Wang,Ye Liu,Xuan Ting Zhang,Dong Qiang Yu

Scientific reports 13:3000 PubMed36810619

2023

Investigation of SAMD1 ablation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bruce Campbell,Lisa M Weber,Sandra J Engle,Terence R S Ozolinš,Patricia Bourassa,Robert Aiello,Robert Liefke

Cell proliferation 56:e13366 PubMed36478274

2022

CD34 cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Soo-Been Jeon,A-Reum Han,Sunghun Lee,Seung Chan Lee,Min Ji Lee,Soon-Jung Park,Sung-Hwan Moon,Ji Yoon Lee

American journal of translational research 14:6712-6725 PubMed36247308

2022

Transplantation of bFGF-transfected bone mesenchymal stem cells on collagen scaffolds promotes the regeneration of injured rat endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Chen,Yingxin Gong,Ninghong Jiang,Jingjing Xiao,Yaping Wang,Limei Chen,Long Sui

Cancer science 113:3148-3160 PubMed35722982

2022

Vascular endothelial growth factor receptor 2 expression and immunotherapy efficacy in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Nakahama,Hiroyasu Kaneda,Masahiko Osawa,Mitsuru Fukui,Motohiro Izumi,Naoki Yoshimoto,Akira Sugimoto,Hiroaki Nagamine,Koichi Ogawa,Yoshiya Matsumoto,Kenji Sawa,Yoko Tani,Shigeki Mitsuoka,Tetsuya Watanabe,Kazuhisa Asai,Tomoya Kawaguchi
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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