Anti-VEGF Receptor 1抗体(ab2350)
Key features and details
- Rabbit polyclonal to VEGF Receptor 1
- Suitable for: WB, IHC-P, ICC/IF, Electron Microscopy
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VEGF Receptor 1抗体
参阅全部 VEGF Receptor 1 一抗 -
描述
兔多克隆抗体to VEGF Receptor 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, Electron Microscopymore details -
种属反应性
与反应: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
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常规说明
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702
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| IHC-P | (2) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Electron Microscopy |
Use at an assay dependent concentration. PubMed: 18647955
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702 |
|
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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Electron Microscopy
Use at an assay dependent concentration. PubMed: 18647955 |
靶标
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功能
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
组织特异性
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2321 Human
- Entrez Gene: 14254 Mouse
- Entrez Gene: 54251 Rat
- Omim: 165070 Human
- SwissProt: P17948 Human
- SwissProt: P35969 Mouse
- SwissProt: P53767 Rat
- Unigene: 594454 Human
see all -
别名
- EC 2.7.10.1 antibody
- FLT 1 antibody
- FLT antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (65)
ab2350 被引用在 65 文献中.
- Li Z et al. Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regeneration. Nat Commun 14:6963 (2023). PubMed: 37907455
- Vinciguerra A et al. Expression, Prognostic Value and Correlation with HPV Status of Hypoxia-Induced Markers in Sinonasal Squamous Cell Carcinoma. J Pers Med 13:N/A (2023). PubMed: 37240937
- Pezzotta A et al. Sirt3 deficiency promotes endothelial dysfunction and aggravates renal injury. PLoS One 18:e0291909 (2023). PubMed: 37816025
- Lin B et al. Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation. Cell Death Discov 8:160 (2022). PubMed: 35379779
- Wang B et al. Astragaloside IV improves angiogenesis under hypoxic conditions by enhancing hypoxia‑inducible factor‑1α SUMOylation. Mol Med Rep 23:N/A (2021). PubMed: 33537820