Anti-VEGF 165A抗体(ab106580)
Key features and details
- Rabbit polyclonal to VEGF 165A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-VEGF 165A抗体
参阅全部 VEGF 165A 一抗 -
描述
兔多克隆抗体to VEGF 165A -
宿主
Rabbit -
特异性
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human VEGF-165 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human VEGF 165A.
Database link: P15692-4 -
阳性对照
- WB: Recombinant human VEGF 165A.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. This purified antibody has been heated to 56°C for 30 minutes. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106580于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
靶标
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功能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
组织特异性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
疾病相关
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
细胞定位
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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数据库链接
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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别名
- glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
- MVCD1 antibody
see all
图片
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Western blot - Anti-VEGF 165A antibody (ab106580)Anti-VEGF 165A antibody (ab106580) at 1/1000 dilution + Recombinant human VEGF 165A
Secondary
Peroxidase conjugated anti-Rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
数据表及文件
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Datasheet download
文献 (3)
ab106580 被引用在 3 文献中.
- Ren R et al. A novel implant surface modification mode of Fe3O4-containing TiO2 nanorods with sinusoidal electromagnetic field for osteoblastogenesis and angiogenesis. Mater Today Bio 19:100590 (2023). PubMed: 36910272
- Chen H et al. VEGF165 gene-modified human umbilical cord blood mesenchymal stem cells protect against acute liver failure in rats. J Gene Med 23:e3369 (2021). PubMed: 34057770
- Yan Y et al. Modeling of diseases of retinal ischemia in vitro: possible participation of autocrine vascular endothelial growth factor signaling. Ophthalmic Res 49:90-9 (2013). PubMed: 23257772
