Anti-VE Cadherin (phospho Y685)抗体(ab119785)
Key features and details
- Rabbit polyclonal to VE Cadherin (phospho Y685)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VE Cadherin (phospho Y685)抗体
参阅全部 VE Cadherin 一抗 -
描述
兔多克隆抗体to VE Cadherin (phospho Y685) -
宿主
Rabbit -
特异性
ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human VE Cadherin (phospho Y685).
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阳性对照
- Human umbilical vein endothelial cells stimulated with pervanadate.
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常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119785于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa). |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
组织特异性
Endothelial tissues and brain. -
序列相似性
Contains 5 cadherin domains. -
翻译后修饰
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
细胞定位
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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数据库链接
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Entrez Gene: 307618 Rat
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: P55284 Mouse
- Unigene: 76206 Human
- Unigene: 21767 Mouse
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别名
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
图片
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Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785)All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution
Lane 1 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min.
Lane 2 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min then treated with
alkaline phosphatase
Predicted band size: 88 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab119785 被引用在 28 文献中.
- Guo J et al. The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cells. Front Cell Dev Biol 11:1076250 (2023). PubMed: 36846596
- Kubra KT et al. Tunicamycin Protects against LPS-Induced Lung Injury. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35215247
- Nyandwi JB et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
- Kubra KT et al. Activating transcription factor 6 protects against endothelial barrier dysfunction. Cell Signal 99:110432 (2022). PubMed: 35933031
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246