Anti-VE Cadherin 抗体 - Intercellular Junction Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

ab33168 staining VE Cadherin in HUV-EC cells. The cells were fixed with 100% methanol (5 min) permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab33168 at 1µg/ml and ab7291 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488) pre-adsorbed at 1/1000 dilution (shown in green) and ab150120 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594) pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

AbReview68027****

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

ab33168 staining VE Cadherin in the endothelial cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde. Samples were incubated with primary antibody (1/100 dilution in PBS + 2.5% BSA + 0.1% triton) for 1 hour at 37°C. Alexa Fluor 594 Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secon was used as the secondary antibody at 4 μg/ml.

This image is courtesy of an Abreview submitted by Kara Shumansky

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

ICC/IF image of VE-Cadherin staining on HUVEC cells using ab33168. The cells were incubated with the primary antibody (ab33168) and the secondary was FITC conjugated anti-rabbit used at 1 : 400. The cells were incubated with only the secondary antibody as a negative control.

This image is courtesy of Stephen Yarwood, Inst Mol, Cell and Sys Bio, United Kingdom

• ICC/IF

AbReview80118****

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

Immunocytochemistry analysis of formaldehyde-fixed human iPSC-EC cells staining with ab33168 at 1/250 dilution. Secondary antibody was Alexa Fluor™ 594 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/500 dilution. The cells were incubated with the primary antibody for 10 hours at 4°C. Blocking was done using 3% BSA for 1 hour at 25°C.

This image is courtesy of an Abreview submitted by Simon Shen

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

ICC/IF image of VE Cadherin stained HUVEC cells. The cells were incubated with the antibody ab33168 at 1/150 dilution (Green). The cells were also stained with Rhodamine phalloidin (Red).

This image is courtesy of Ana Kasirer-Friede, Univ California-San Diego, Dept. Of Medicine, United States

• WB

Lab

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

Gel type : MOPS Blocking buffer : 2% BSA

All lanes:

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL

Lane 1:

HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

Lane 2:

Mouse lung tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,70 kDa

false

Exposure time: 1min

• WB

Lab

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

The observed band for Cadherin 5 has a higher molecular weight of 115kDa due to glycosylation of the protein.

The immunogen used to raise this antibody has 89% homology with Cadherin 18, 88kDa , which we believe is the additional observed band at 117kDa, again due to glycosylation of the protein.

All lanes:

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL

All lanes:

HUVEC Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 88 kDa

Observed band size: 115 kDa,117 kDa,45 kDa

true

Exposure time: 1min

• WB

Lab

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

The band we observe at 115 kDa is believed to be the glycosylated form of the protein.

All lanes:

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL

All lanes:

HUVEC Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa,55 kDa

true

Exposure time: 1min

• WB

Lab

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (AB33168)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

The band we observe at 115 kDa is believed to be the glycosylated form of the protein.

All lanes:

Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL

All lanes:

HUVEC Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 88 kDa

Observed band size: 120 kDa

true

Exposure time: 1min