AB181315

重组Anti-VCAM1抗体[EPR17010-83]

Anti-VCAM1 antibody [EPR17010-83]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal VCAM1 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 3 publications.

查看别名

CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1

4 Images

Supplier Data

Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] (AB181315)

VCAM1 was immunoprecipitated from 1 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab181315 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab181315 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10μg (Input).

Lane 2 : ab181315 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181315 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] (ab181315)

Predicted band size: 81 kDa

false

Lab

Western blot - Anti-VCAM1 antibody [EPR17010-83] (AB181315)

Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

Blocking/Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/1000 dilution

Lane 1:

LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate at 20 µg

Lane 2:

bEnd.3 (Mouse brain endothelioma) whole cell lysate at 20 µg

Lane 3:

bEnd.3 (Mouse brain endothelioma) treated with 10 μg/ml LPS for 24 h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa,45 kDa

false

Exposure time: 50s

Supplier Data

Western blot - Anti-VCAM1 antibody [EPR17010-83] (AB181315)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2 : 15 seconds.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Mouse bone marrow lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa

false

Supplier Data

Western blot - Anti-VCAM1 antibody [EPR17010-83] (AB181315)

Blocking/Dilution buffer : 5% NFDM/TBST.

Mouse VCAM1 active protein contains aa1-698.

All lanes:

Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/5000 dilution

All lanes:

Mouse VCAM1 active protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 81 kDa

Observed band size: 100 kDa

false

Exposure time: 3min

不同偶联物与剂型 (2)

Carrier free

Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free
Carrier free

Anti-VCAM1 antibody [EPR17010-83] - BSA and Azide free (Capture)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17010-83

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.</p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.

See full target information Vcam1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:185-198 PubMed39735673

2024

lncRNA SNHG4 enhanced gastric cancer progression by modulating miR-409-3p/CREB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouyang Cheng,Yuchen Hua,Yang Cao,Jun Qin

Experimental and therapeutic medicine 25:240 PubMed37114177

2023

Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhao,Huizhen Wei,Jun Lu,Wenjun Sha,Dusang Sun,Ting Pan,Tao Lei

Cell cycle (Georgetown, Tex.) 18:274-287 PubMed30661459

2019

IL-17 produced by Th17 cells alleviates the severity of fungal keratitis by suppressing CX43 expression in corneal peripheral vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Hong Qin,Xiang Ma,Shi-Feng Fang,Zhen-Zhen Zhang,Jian-Min Lu

View all publications

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