重组Anti-Vasopressin抗体[EPR20602]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (AB213708)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected withVasopressin expression vector containing a myc-His-tag^® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Co-stained with Alexa Fluor^® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.

• IP

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Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (AB213708)

Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate 10 μg (Input).

Lane 2 : ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)

Predicted band size: 17 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (AB213708)

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID : 22822466, PMID : 17437647, PMID : 18773953). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (AB213708)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (AB213708)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID : 22822466, PMID : 17437647, PMID : 18773953). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (AB213708)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• WB

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Western blot - Anti-Vasopressin antibody [EPR20602] (AB213708)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

true

Exposure time: 1s