AB196487

Anti-VAPB抗体

Name: VAPB抗体 (ab196487)| Abcam中文官网
Brand: Abcam Limited
SKU: AB196487
Price: 4744 CNY
Availability: InStock

Anti-VAPB antibody

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(3 Publications)

Rabbit Polyclonal VAPB antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAPB.

查看别名

UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

Immunofluorescence analysis of HeLa cells labeling VAPB (green) with ab196487 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-VAPB antibody (AB196487)

All lanes:

Western blot - Anti-VAPB antibody (ab196487) at 1/500 dilution

Lane 1:

BT474 cell extract

Lane 2:

SW480 cell extract

Lane 3:

HepG2 cell extract

Lane 4:

H460 cell extract

Lane 5:

Raji cell extract

Lane 6:

Mouse brain tissue extract

Lane 7:

Mouse intestine tissue extract

Predicted band size: 27 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human VAPB. The exact immunogen used to generate this antibody is proprietary information.

O95292

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

VAMP-associated protein B (VAPB) also known as VAP-B is a protein with a mass of approximately 27 kDa. It plays a role in membrane trafficking and lipid transport. VAPB accomplishes this by interacting with various vesicle-associated membrane protein (VAMP) family members. It is expressed in multiple tissues including the brain liver and muscle. VAPB localizes to the endoplasmic reticulum where it facilitates its mechanical functions.

Biological function summary

The protein assists in maintaining cellular homeostasis by influencing lipid transport and membrane composition. VAPB forms part of a larger protein complex that includes other lipid-binding proteins and is essential for the regulation of lipid-dependent signaling pathways. This complex allows VAPB to perform its biological functions effectively within cells.

Pathways

VAPB participates in critical pathways like the unfolded protein response (UPR) and ER-associated degradation (ERAD). These pathways ensure proper protein folding and degradation. VAPB interacts with other important proteins such as VAMP family members to convey signals and manage lipid distribution. Disruptions in these pathways can affect cellular functions evidencing VAPB's integral role.

VAPB has strong connections to certain neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Mutations in the VAPB gene have been linked to the familial form of ALS impacting the protein's function and leading to disease progression. VAPB associates with proteins like TDP-43 which also play a role in neurodegenerative disease mechanisms establishing its critical involvement in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). Interacts with STARD3 in a FFAT motif phosphorylation dependent manner (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity (PubMed : 16891305, PubMed : 20940299). Involved in cellular calcium homeostasis regulation (PubMed : 22131369).

See full target information VAPB

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112883 PubMed37498742

2023

COPI-regulated mitochondria-ER contact site formation maintains axonal integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Maddison,Bilal Malik,Leonardo Amadio,Dana M Bis-Brewer,Stephan Züchner,Owen M Peters,Gaynor A Smith

Science advances 6:eaax9093 PubMed32494695

2020

Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Rimessi,Chiara Pozzato,Lorenzo Carparelli,Alice Rossi,Serena Ranucci,Ida De Fino,Cristina Cigana,Anna Talarico,Mariusz R Wieckowski,Carla M P Ribeiro,Claudio Trapella,Giacomo Rossi,Giulio Cabrini,Alessandra Bragonzi,Paolo Pinton

PloS one 14:e0214198 PubMed30921354

2019

Phenotypic subgrouping and multi-omics analyses reveal reduced diazepam-binding inhibitor (DBI) protein levels in autism spectrum disorder with severe language impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Chatravee Pichitpunpong,Surangrat Thongkorn,Songphon Kanlayaprasit,Wasana Yuwattana,Waluga Plaingam,Siriporn Sangsuthum,Wan Mohd Aizat,Syarul Nataqain Baharum,Tewin Tencomnao,Valerie Wailin Hu,Tewarit Sarachana

View all publications

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Anti-VAPB抗体

Anti-VAPB antibody

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

Western blot - Anti-VAPB antibody (AB196487)