重组Anti-VAPA抗体[EPR13589(B)] - N-terminal (ab181067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13589(B)] to VAPA - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-VAPA抗体[EPR13589(B)] - N-terminal
参阅全部 VAPA 一抗 -
描述
兔单克隆抗体[EPR13589(B)] to VAPA - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, K562, HEK293, HepG2 cell lysates. Human fetal colon and Human ovarian carcinoma tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13589(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181067于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in vesicle trafficking. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
细胞定位
Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 516024 Cow
- Entrez Gene: 9218 Human
- Entrez Gene: 30960 Mouse
- Entrez Gene: 100173641 Orangutan
- Entrez Gene: 58857 Rat
- Omim: 605703 Human
- SwissProt: Q0VCY1 Cow
- SwissProt: Q9P0L0 Human
see all -
别名
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
see all
图片
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All lanes : Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemical analysis of Human fetal colon tissue, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Human ovarian carcinoma, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab181067 被引用在 3 文献中.
- Lu M et al. The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Sci Adv 6:N/A (2020). PubMed: 33328230
- Papadopoulou AA et al. Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Rep 20:e46451 (2019). PubMed: 32048434
- Papadopoulou AA et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281