AB181067

重组Anti-VAPA抗体[EPR13589(B)] - N-terminal

Name: VAPA抗体[EPR13589(B)] - N-terminal (ab181067)| Abcam中文官网
Brand: Abcam Limited
SKU: AB181067
Price: 2442 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-VAPA antibody [EPR13589(B)] - N-terminal

RabMAb
Recombinant
了解详情

(2 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal VAPA antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

查看别名

VAP33, VAPA, Vesicle-associated membrane protein-associated protein A, VAMP-A, VAMP-associated protein A, VAP-A, 33 kDa VAMP-associated protein, VAP-33

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Immunohistochemical analysis of Human fetal colon tissue staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

Immunohistochemical analysis of Human ovarian carcinoma staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

All lanes:

Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HEK293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-VAPA antibody [EPR13589(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13589(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein-associated protein A (VAPA) also known as VAP-A is an integral membrane protein involved in intracellular membrane trafficking. It has a molecular weight of approximately 33 kDa and is expressed in multiple tissues including the brain liver and muscles. Mechanically VAPA functions in the process of vesicle docking and fusion at membranes which is essential for the trafficking of proteins and lipids within cells. This protein is also a part of the endoplasmic reticulum (ER) and contributes to the formation of membrane contact sites between the ER and other organelles.

Biological function summary

In the cellular environment VAPA plays an important role in maintaining homeostasis by regulating lipid transport and metabolism. It forms a complex with several other proteins such as OSBP (oxysterol-binding protein) and CERT (ceramide transport protein) which facilitates the exchange of lipids between the ER and the Golgi apparatus. This interaction underlines VAPA’s role in lipid droplet dynamics and the synthesis of phosphatidylinositols which are critical for cell signaling and membrane integrity.

Pathways

The involvement of VAPA is significant in lipid metabolism and membrane trafficking pathways. It interacts with proteins like VAMP (vesicle-associated membrane proteins) and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes which are important for exocytosis and endocytosis processes. VAPA's interaction in these pathways supports the movement of materials across cellular membranes and its regulation of lipid exchange impacts the signaling pathways influencing cell growth and differentiation.

Abnormal VAPA function relates to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and some forms of motor neuron disease. In such conditions VAPA’s interaction with proteins like TDP-43 (TAR DNA-binding protein 43) becomes disrupted leading to protein aggregation and neuronal toxicity. Additionally disruptions in VAPA-associated lipid transport are linked to metabolic disorders like type 2 diabetes highlighting its critical role in proper cellular communication and metabolic balance.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). In addition, recruited to the plasma membrane through OSBPL3 binding (PubMed : 25447204). The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 25447204). With OSBPL3, may regulate ER morphology (PubMed : 16143324). May play a role in vesicle trafficking (PubMed : 11511104, PubMed : 19289470).

See full target information VAPA

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:5451-5461 PubMed39247600

2024

circTP63 promotes prostate cancer progression via miR-421/VAMP associated protein A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Xu,Siwei Xu,Weihui Liu,Jiabi Chen,Longbo Cai,Wei Zhuang

Nature cell biology 26:378-392 PubMed38429475

2024

Combinatorial selective ER-phagy remodels the ER during neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa J Hoyer,Cristina Capitanio,Ian R Smith,Julia C Paoli,Anna Bieber,Yizhi Jiang,Joao A Paulo,Miguel A Gonzalez-Lozano,Wolfgang Baumeister,Florian Wilfling,Brenda A Schulman,J Wade Harper

The Journal of cell biology 222: PubMed37737955

2023

Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Pellegrino,Beren Aylan,Claudio Bussi,Antony Fearns,Elliott M Bernard,Natalia Athanasiadi,Pierre Santucci,Laure Botella,Maximiliano G Gutierrez

Proceedings of the National Academy of Sciences of the United States of America 120:e2219790120 PubMed37307450

2023

Dynamic interactome of the MHC I peptide loading complex in human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Barends,Nicole Koller,Christian Schölz,Verónica Durán,Berislav Bošnjak,Jennifer Becker,Marius Döring,Hanna Blees,Reinhold Förster,Ulrich Kalinke,Robert Tampé

Science advances 6: PubMed33328230

2020

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Lu,Francesca W van Tartwijk,Julie Qiaojin Lin,Wilco Nijenhuis,Pierre Parutto,Marcus Fantham,Charles N Christensen,Edward Avezov,Christine E Holt,Alan Tunnacliffe,David Holcman,Lukas Kapitein,Gabriele S Kaminski Schierle,Clemens F Kaminski

EMBO reports 20:e46451 PubMed32048434

2019

Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alkmini A Papadopoulou,Stephan A Müller,Torben Mentrup,Merav D Shmueli,Johannes Niemeyer,Martina Haug-Kröper,Julia von Blume,Artur Mayerhofer,Regina Feederle,Bernd Schröder,Stefan F Lichtenthaler,Regina Fluhrer

EMBO reports 20: PubMed30733281

2019

Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

View all publications

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重组Anti-VAPA抗体[EPR13589(B)] - N-terminal