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Signal Transduction Protein Trafficking Vesicle Transport Coat Proteins

Anti-VAPA抗体(ab176995)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)
  • Western blot - Anti-VAPA antibody (ab176995)

Key features and details

  • Rabbit polyclonal to VAPA
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-VAPA抗体
    参阅全部 VAPA 一抗
  • 描述

    兔多克隆抗体to VAPA
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant fragment corresponding to Human VAPA aa 18-204. BC002992
    Sequence:

    DPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLR


    Database link: Q9P0L0
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • NIH-3T3, 293, HepG2, Fetal brain, fetal stomach lysates. Coloon cancer tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Lyophilized:200 ul sterile water
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human VAPA protein (ab93462)
  • Related Products

    • Recombinant Human VAPA protein (ab93462)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176995于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/100 - 1/500.
WB (2)
1/200 - 1/1000. Predicted molecular weight: 28 kDa.
说明
IHC-P
1/100 - 1/500.
WB
1/200 - 1/1000. Predicted molecular weight: 28 kDa.

靶标

  • 功能

    May play a role in vesicle trafficking.
  • 组织特异性

    Ubiquitous.
  • 序列相似性

    Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.
  • 细胞定位

    Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Target information above from: UniProt accession Q9P0L0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 516024 Cow
    • Entrez Gene: 9218 Human
    • Entrez Gene: 30960 Mouse
    • Entrez Gene: 58857 Rat
    • Omim: 605703 Human
    • SwissProt: Q0VCY1 Cow
    • SwissProt: Q9P0L0 Human
    • SwissProt: Q9WV55 Mouse
    • SwissProt: Q9Z270 Rat
    • Unigene: 165195 Human
    • Unigene: 266767 Mouse
    • Unigene: 162275 Rat
    see all
  • 别名

    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)

    Immunohistochemical staining of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling VAPA with ab176995 at 1/100 dilution showing cytoplasmic staining

  • Western blot - Anti-VAPA antibody (ab176995)
    Western blot - Anti-VAPA antibody (ab176995)
    All lanes : Anti-VAPA antibody (ab176995) at 1/500 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : Human fetal stomach lysate

    Predicted band size: 28 kDa

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab176995?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab176995 尚未被引用在任何文献中。

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-VAPA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Ixodes scapularis Cell lysate - whole cell (ISE6)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
ISE6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 23 2020

Western blot abreview for Anti-VAPA antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 20 2018

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