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重组RabMAb

重组Anti-VAP1抗体[EPR28748-59] (ab317620)

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Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
  • Anti-VAP1 antibody [EPR28748-59] (ab317620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28748-59] to VAP1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-VAP1抗体[EPR28748-59]
    参阅全部 VAP1 一抗

  • 描述

    兔单克隆抗体[EPR28748-59] to VAP1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human lung, Human skeletal muscle, Human colon, Human liver, Mouse lung, Mouse skeletal muscle, Mouse colon, Rat lung, Rat skeletal muscle and Rat colon lysates. IHC-P: Human lung, Human colon, Human skeletal muscle, Mouse colon and Rat lung tissues.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317620于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 85 kDa.
说明
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 85 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

  • 功能

    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.

  • 组织特异性

    Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.

  • 序列相似性

    Belongs to the copper/topaquinone oxidase family.

  • 翻译后修饰

    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
    • AOC3 antibody
    • AOC3_HUMAN antibody
    • Copper amine oxidase antibody
    • HPAO antibody
    • Membrane copper amine oxidase antibody
    • Membrane primary amine oxidase antibody
    • Semicarbazide sensitive amine oxidase antibody
    • Semicarbazide-sensitive amine oxidase antibody
    • SSAO antibody
    • VAP-1 antibody
    • Vascular adhesion protein 1 antibody
    • VP97 antibody
    see all

图片

  • Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    All lanes : Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

    Lane 1 : Human lung tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate
    Lane 3 : Human colon tissue lysate
    Lane 4 : Human liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle (PMID: 23349812).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 15 seconds; Lane 4: 81 seconds.

  • Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    All lanes : Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Mouse colon tissue lysate
    Lane 4 : Rat lung tissue lysate
    Lane 5 : Rat skeletal muscle tissue lysate
    Lane 6 : Rat colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle (PMID: 23349812).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of human lung.
    The section was incubated with ab317620 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle and endothelium of human colon (PMID: 26912327).
    The section was incubated with ab317620 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of human skeletal muscle (PMID: 20154208).
    The section was incubated with ab317620 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle and endothelium of mouse colon.
    The section was incubated with ab317620 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of rat lung.
    The section was incubated with ab317620 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Anti-VAP1 antibody [EPR28748-59] (ab317620)
    Anti-VAP1 antibody [EPR28748-59] (ab317620)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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