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重组RabMAb

重组Anti-VAMP2抗体[EPR20818] - BSA and Azide free (ab226863)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
  • Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
  • Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20818] to VAMP2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-VAMP2抗体[EPR20818] - BSA and Azide free
    参阅全部 VAMP2 一抗

  • 描述

    兔单克隆抗体[EPR20818] to VAMP2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human kidney tissue.

  • 常规说明

    ab226863 is the carrier-free version of ab215721.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226863于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.

  • 组织特异性

    Nervous system and skeletal muscle.

  • 序列相似性

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.

  • 细胞定位

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse cerebrum. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215721).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat cerebrum. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215721).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
    Immunoprecipitation - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

    VAMP2 was immunoprecipitated from 0.35 mg mouse brain lysate with ab215721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate 10 μg (Input).

    Lane 2: ab215721 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab215721 in mouse brain lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215721).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human kidney. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215721).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)
    Anti-VAMP2 antibody [EPR20818] - BSA and Azide free (ab226863)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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