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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-VAMP2抗体(ab3347)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (43)

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Western blot - Anti-VAMP2 antibody (ab3347)

    Key features and details

    • Rabbit polyclonal to VAMP2
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-VAMP2 antibody [EPR12790] (ab181869)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-VAMP2抗体
      参阅全部 VAMP2 一抗
    • 描述

      兔多克隆抗体to VAMP2
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Rat
      预测可用于: Rhesus monkey
    • 免疫原

      Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
      Sequence:

      MSATAATVPPAAPAGEGG


      (Peptide available as ab4958)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • WB: Mouse brain and Rat brain lysates
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Mouse VAMP2 protein (His tag) (ab222979)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3347于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
    说明
    WB
    Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    靶标

    • 功能

      Involved in the targeting and/or fusion of transport vesicles to their target membrane.
    • 组织特异性

      Nervous system and skeletal muscle.
    • 序列相似性

      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • 细胞定位

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
    • Target information above from: UniProt accession P63027 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 22318 Mouse
      • Entrez Gene: 24803 Rat
      • SwissProt: P63044 Mouse
      • SwissProt: P63045 Rat
      • Unigene: 28643 Mouse
      • Unigene: 12939 Rat
      • 别名

        • FLJ11460 antibody
        • RATVAMPB antibody
        • RATVAMPIR antibody
        • SYB antibody
        • SYB2 antibody
        • Synaptobrevin 2 antibody
        • Synaptobrevin-2 antibody
        • VAMP 2 antibody
        • VAMP-2 antibody
        • Vamp2 antibody
        • VAMP2_HUMAN antibody
        • Vesicle associated membrane protein 2 antibody
        • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
        • Vesicle-associated membrane protein 2 antibody
        see all

      图片

      • Western blot - Anti-VAMP2 antibody (ab3347)
        Western blot - Anti-VAMP2 antibody (ab3347)
        All lanes : Anti-VAMP2 antibody (ab3347) at 1 µg/ml

        Lane 1 : Mouse brain lysate
        Lane 2 : Rat brain lysate
        Lane 3 : Mouse lung lysate
        Lane 4 : Rat lung lysate
        Lane 5 : Rat liver lysate
        Lane 6 : Rat kidney lysate

        Lysates/proteins at 30 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

        Predicted band size: 19 kDa

      实验方案

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (43)

      发表研究结果有使用 ab3347?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3347 被引用在 43 文献中.

      • Wilhelmi I  et al. The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic ß-cells. Mol Metab 45:101151 (2021). PubMed: 33359402
      • Ivanova D  et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
      • Rong X  et al. Enalapril attenuates endoplasmic reticulum stress and mitochondrial injury induced by myocardial infarction via activation of the TAK1/NFAT pathway in mice. Exp Ther Med 19:972-980 (2020). PubMed: 32010259
      • Tanaka M  et al. Enhanced phosphorylation of PERK in primary cultured neurons as an autonomous neuronal response to prion infection. PLoS One 15:e0234147 (2020). PubMed: 32479530
      • Ke YD  et al. CNS cell type-specific gene profiling of P301S tau transgenic mice identifies genes dysregulated by progressive tau accumulation. J Biol Chem 294:14149-14162 (2019). PubMed: 31366728
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-VAMP2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      2 µg
      Gel Running Conditions
      Non-reduced Denaturing
      Sample
      Rat Tissue lysate - whole (hippocampus tissue lysate)
      Specification
      hippocampus tissue lysate
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 07 2014

      Immunohistochemistry (Frozen sections) abreview for Anti-VAMP2 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Karine Thibault

      Verified customer

      提交于 Apr 14 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-VAMP2 antibody

      Good
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (hippocampal neuron)
      Specification
      hippocampal neuron
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 2%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Chan Choo Yap

      Verified customer

      提交于 Nov 27 2006

      Question

      In hopes of answering your questions:

      1) the problem is we are not seeing any bands even in a control sample prepared from kidney cortex.
      2) We are using protein samples from IMCD3 cell lystates. These were prepared using a RIPA lysis buffer.
      3) The primary antibody is being incubated overnight at 4 degrees Celsius.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 05 2012

      Answer

      Thank you for your reply and the additional information.

      Please let me know your order or PO number. If the antibody was purchased within the last 6 months, our Abpromise guarantee applies and I'd be happy to offer you either a free of charge replacement, a credit or a refund.

      Please let me know which option would be best for you.

      I look forward to hear back and resolve this case for you.

      Read More

      Abcam Scientific Support

      回复于 Nov 05 2012

      Question

      We have attempted to use your Anti-VAMP2 antibody (ab3347) for Western Blotting. We have unfortunately had no success working with a 1:500 dilution. Is there a suggested protocol that you may be able to offer?

      Here are some highlights of our procedure:

      - 100ug protein loaded
      - 15 minute denaturation at 75 degrees Celsius
      - 1 Hour block with 10% Non Fat Dry Skim Milk (NFDSM)
      - 1:500 Anti-VAMP2 in 10% NFDSM
      - 90 min 1:2000 Secondary
      - 30 min ECL development

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2012

      Answer

      Thank you for contacting us.
      I am sorry to hear that the antibody is not working as expected.

      I have copied the WB protocol below.
      Also,
      I have a few more questions in order to understand the issue better:
      1) What is the exact problem (e.g. no bands, high background)?
      2) What type of samples have you used and how were they prepared?
      3) How long did you incubate with the primary antibody?



      Western Blot Protocol

      Polyacrylamide gel electrophoresis and blottingAdd an appropriate amount of electrophoresis sample buffer (1X = 125mM Tris-HCl pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, and 1% beta-mercaptoethanol) to all samples.
      Heat to 95°C for 3-5 minutes.
      Load 5-100 ug total protein in a volume that is appropriate for the size of the wells.
      Electrophorese proteins for the appropriate time according to the manufacturers instructions.
      Transfer proteins from the gel to a suitable membrane (e.g. nitrocellulose, PVDF, etc.) following the manufacturers protocol for transfer.
      High molecular weight proteins (>120 kDa) can be wet transferred more efficiently if transfer time is increased and 0.05% SDS is included in the transfer buffer.


      BlockingRemove the filter from the transfer apparatus and rinse in PBST/TBST to remove loose acrylamide.
      Transferred proteins can be visualized by staining the membrane for 15-30 minutes with Ponceau S.
      Remove stain from filter by washing with PBST/TBST.
      Place filter into blocking solution.
      Block for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


      Incubation with primary antibodyDecant the blocking buffer and add the antibody, diluted in blocking buffer as suggested in the product description sheet.
      Incubate with agitation for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


      Incubation with secondary antibodyWash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.
      Decant the wash solution and add AffiniClear HRP-conjugated secondary antibody, diluted in 5% non-fat dry milk in wash buffer.
      Incubate for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.
      Decant the antibody conjugate and wash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.


      Substrate incubation (ECL)Decant washing buffer and place the blot in a plastic bag or clean tray containing the development working solution (0.125 ml/cm2) for 1-5 minutes.
      Agitate bag or tray to cover the surface of the membrane.
      Remove the blot from the bag or tray and place it between two pieces of write-on transparency film.
      Smooth over the covered blot to remove air bubbles and excess substrate.
      Expose to X-ray film or any sensitive screen. (Check manufacturer's instructions for specific ECL reagents and procedures.)


      I look forward to hear back from you and assist you further.

      Read More

      Abcam Scientific Support

      回复于 Nov 02 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
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