Anti-Vaccinia Virus抗体(ab26853)
Key features and details
- Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA
- Reacts with: Vaccinia virus
- Isotype: IgG
概述
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产品名称
Anti-Vaccinia Virus抗体
参阅全部 Vaccinia Virus 一抗 -
描述
兔多克隆抗体to Vaccinia Virus -
宿主
Rabbit -
经测试应用
适用于: ELISAmore details -
种属反应性
与反应: Vaccinia virus -
免疫原
Synthetic peptide corresponding to Vaccinia Virus (N terminal). Containing amino acids on the predicted N terminal of fusion protein (A27L) in vaccinia virus.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/5000.
|
| 说明 |
|---|
|
ELISA
1/5000. |
靶标
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相关性
Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process. -
别名
- Orthopoxvirus antibody
图片
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ELISA - Anti-Vaccinia Virus antibody (ab26853)ELISA method:
Antigen is coated on EIA strips at 1µg per well.
Add 200µl blocking buffer then wash wells with PBST.
Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
Incubate antibody for 1 hour.
Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
Wash plates and add substrate to develop color for 5 minutes.
Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
Titer ~ 1/437000.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
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