Anti-V5 tag抗体[SV5-Pk1] (ab27671)
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.cn/ps/products/27/ab27671/Images/ab27671-380968-anti-v5-tag-antibody-western-blot-nih3t3-cell-lysate.jpg "Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)")
Key features and details
- Mouse monoclonal [SV5-Pk1] to V5 tag
- Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIA
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-V5 tag抗体[SV5-Pk1]
参阅全部 V5 tag 一抗 -
描述
小鼠单克隆抗体[SV5-Pk1] to V5 tag -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details -
种属反应性
与反应: Species independent -
免疫原
Tissue, cells or virus corresponding to V5 tag. Paramyxovirus Simian-Virus 5 (SV5)
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表位
ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. -
阳性对照
- WB: Transfected NIH/3T3 and COS-7 cell lysates.
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常规说明
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
This antibody was purified from tissue culture supernatant by affinity chromatography. -
Primary antibody说明
Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope. -
克隆
单克隆 -
克隆编号
SV5-Pk1 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
1/1000 - 1/5000.
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|
| IP |
Use at an assay dependent concentration.
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|
| WB | (8) |
1/1000 - 1/5000.
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| RIA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
1/1000 - 1/5000. |
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IP
Use at an assay dependent concentration. |
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WB
1/1000 - 1/5000. |
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RIA
Use at an assay dependent concentration. |
靶标
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相关性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
别名
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
图片
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Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)All lanes : Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1-2 : Transfected NIH/3T3 cell lysate
Lane 3 : Not transfected NIH/3T3 cell lysate
Secondary
All lanes : HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1 secondCells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
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![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.cn/ps/products/27/ab27671/Images/ab27671-318649-anti-v5-tag-antibody-sv5-pk1-western-blot.jpg)
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (148)
ab27671 被引用在 148 文献中.
- Sun C et al. BmCaspase-8-like regulates autophagy by suppressing BmDREDD-mediated cleavage of BmATG6. Insect Sci 30:365-374 (2023). PubMed: 36044542
- Sollis E et al. Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions. Hum Mol Genet 32:1497-1510 (2023). PubMed: 36579832
- Bandyopadhyay S et al. A Biological OR(XNOR) Logic Gate Couples Carbon Source and Transgene Expression Switching in a Komagataella phaffii (Pichia pastoris) Strain Co-producing Process-Enhancing Lipase and a Virus-like Particle (VLP) Vaccine. ACS Synth Biol 12:657-663 (2023). PubMed: 36848292
- Pilat JM et al. SELENOP modifies sporadic colorectal carcinogenesis and WNT signaling activity through LRP5/6 interactions. J Clin Invest 133:N/A (2023). PubMed: 37166989
- Pierga A et al. Spatacsin regulates directionality of lysosome trafficking by promoting the degradation of its partner AP5Z1. PLoS Biol 21:e3002337 (2023). PubMed: 37871017