AB206571

重组Anti-V5 tag抗体[SV5-P-K]

Anti-V5 tag antibody [SV5-P-K]

Recombinant
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(6 Publications)

Rat Recombinant Monoclonal V5 tag antibody. Suitable for IP, ELISA, WB, RIA, ICC/IF, IHC-Fr and reacts with Tag samples. Cited in 6 publications.

查看别名

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

1 Images

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206571)

ELISA

Supplier Data

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206571)

Plate coated with 5ug/ml of multi-tag protein.

ab206751 loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

SV5-P-K

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

应用

IHC-Fr, ELISA, ICC/IF, RIA, WB, IP

applications

特异性

ab206571 recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the Paramyxo Virus simian virus 5.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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产品详情

This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.02% Proclin 300 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information V5 tag

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103617 PubMed39891917

2025

Protocol for assessing the clogging of the mitochondrial translocase of the outer membrane by precursor proteins in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

John Kim,Hilla Weidberg

The EMBO journal 44:1249-1273 PubMed39806097

2025

E3 ubiquitin ligase CHIP facilitates cAMP and cGMP signalling cross-talk by polyubiquitinating PDE9A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Hao,Zhengwei Hu,Mengjie Li,Shuo Zhang,Mibo Tang,Chenwei Hao,Shasha Qi,Yuanyuan Liang,Michael F Almeida,Kaitlan Smith,Chunyan Zuo,Yanmei Feng,Mengnan Guo,Dongrui Ma,Shuangjie Li,Zhiyun Wang,Yuemeng Sun,Zhifen Deng,Chengyuan Mao,Zongping Xia,Yong Jiang,Yanxia Gao,Yuming Xu,Jonathan C Schisler,Changhe Shi

Cell 187:5081-5101.e19 PubMed38996528

2024

Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanyun Xu,Zhuoran Li,Cheng Lyu,Yixin Hu,Colleen N McLaughlin,Kenneth Kin Lam Wong,Qijing Xie,David J Luginbuhl,Hongjie Li,Namrata D Udeshi,Tanya Svinkina,D R Mani,Shuo Han,Tongchao Li,Yang Li,Ricardo Guajardo,Alice Y Ting,Steven A Carr,Jiefu Li,Liqun Luo

Molecular cancer 22:135 PubMed37580748

2023

Correction: Reprogramming anchorage dependency by adherent‑to‑suspension transition promotes metastatic dissemination.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunbin D Huh,Yujin Sub,Jongwook Oh,Ye Eun Kim,Ju Young Lee,Hwa-Ryeon Kim,Soyeon Lee,Hannah Lee,Sehyung Pak,Sebastian E Amos,Danielle Vahala,Jae Hyung Park,Ji Eun Shin,So Yeon Park,Han Sang Kim,Young Hoon Roh,Han-Woong Lee,Kun-Liang Guan,Yu Suk Choi,Joon Jeong,Junjeong Choi,Jae-Seok Roe,Heon Yung Gee,Hyun Woo Park

mBio 11: PubMed32665270

2020

Convergent Evolution of HLA-C Downmodulation in HIV-1 and HIV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Hopfensperger,Jonathan Richard,Christina M Stürzel,Frederic Bibollet-Ruche,Richard Apps,Marie Leoz,Jean-Christophe Plantier,Beatrice H Hahn,Andrés Finzi,Frank Kirchhoff,Daniel Sauter

JCI insight 3: PubMed29669933

2018

Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ashraf Ul Kabir,Tae-Jin Lee,Hua Pan,Jeffrey C Berry,Karen Krchma,Jun Wu,Fang Liu,Hee-Kyoung Kang,Kristina Hinman,Lihua Yang,Samantha Hamilton,Qingyu Zhou,Deborah J Veis,Robert P Mecham,Samuel A Wickline,Mark J Miller,Kyunghee Choi

View all publications

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