Anti-V5 tag抗体(ab9116)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: WB, ELISA, IP, IHC-P
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-V5 tag抗体
参阅全部 V5 tag 一抗 -
描述
兔多克隆抗体to V5 tag -
宿主
Rabbit -
特异性
ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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经测试应用
适用于: WB, ELISA, IP, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Parainfluenza virus 5 V5 tag aa 50-150 conjugated to keyhole limpet haemocyanin. (V5 epitope)
Database link: P11208 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg . -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) |
Use a concentration of 0.05 - 0.5 µg/ml.
|
| ELISA |
Use at an assay dependent concentration.
|
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| IP | (2) |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 0.5 - 5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.05 - 0.5 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 0.5 - 5 µg/ml. |
靶标
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相关性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
别名
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
图片
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Western blot - Anti-V5 tag antibody (ab9116)This image is courtesy of an anonymous AbreviewAll lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
Lanes 1-3 : Cells transfected with v5-tagged protein.
Lanes 4-5 : Untransfected cells.
Secondary
Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (166)
ab9116 被引用在 166 文献中.
- Naqvi S et al. Precise modulation of transcription factor levels identifies features underlying dosage sensitivity. Nat Genet 55:841-851 (2023). PubMed: 37024583
- Wang B et al. A crucial role for dynamic expression of components encoding the negative arm of the circadian clock. Nat Commun 14:3371 (2023). PubMed: 37291101
- Olajide OM et al. Evolutionarily conserved amino acids in MHC-II mediate bat influenza A virus entry into human cells. PLoS Biol 21:e3002182 (2023). PubMed: 37410798
- Besschetnova A et al. Demethylation of EHMT1/GLP Protein Reprograms Its Transcriptional Activity and Promotes Prostate Cancer Progression. Cancer Res Commun 3:1716-1730 (2023). PubMed: 37663929
- Bosnakovski D et al. Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein. iScience 26:107823 (2023). PubMed: 37744032