Anti-USP6抗体(ab224725)
Key features and details
- Rabbit polyclonal to USP6
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-USP6抗体 -
描述
兔多克隆抗体to USP6 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment corresponding to Human USP6 aa 1100-1400.
Database link: P35125-1 -
阳性对照
- WB: Rat kidney tissue lysate. IHC: Human ovarian cancer tissue. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224725于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| WB |
1/500 - 1/5000.
|
| 说明 |
|---|
|
IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
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WB
1/500 - 1/5000. |
靶标
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功能
Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation. -
组织特异性
Testis specific. Expressed in various cancer cell lines. -
疾病相关
Note=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation. -
序列相似性
Belongs to the peptidase C19 family.
Contains 1 Rab-GAP TBC domain. -
结构域
The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6. -
翻译后修饰
Monubiquitinated; ubiquitination is calmodulin and calcium dependent. -
细胞定位
Cell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 9098 Human
- Omim: 604334 Human
- SwissProt: P35125 Human
- Unigene: 448851 Human
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别名
- Deubiquitinating enzyme 6 antibody
- HRP1 antibody
- Proto-oncogene TRE-2 antibody
see all
图片
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Western blot - Anti-USP6 antibody (ab224725)Anti-USP6 antibody (ab224725) at 1/500 dilution + rat kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Observed band size: 159 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP6 antibody (ab224725)Paraffin embedded human ovarian cancer tissue stained for USP6 with ab224725 (1/100 dilution) in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-USP6 antibody (ab224725)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for USP6 (green) using ab224725 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224725 被引用在 1 文献中.
- Jia L et al. Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression. BMC Cancer 23:1042 (2023). PubMed: 37904094