Anti-USP51抗体(ab121147)
Key features and details
- Rabbit polyclonal to USP51
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-USP51抗体 -
描述
兔多克隆抗体to USP51 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Pig, Gorilla
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免疫原
Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).
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阳性对照
- IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性
Expressed in prostate, brain, lung, aorta and kidney. -
序列相似性
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 158880 Human
- SwissProt: Q70EK9 Human
- Unigene: 40061 Human
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别名
- Deubiquitinating enzyme 51 antibody
- Ubiquitin carboxyl-terminal hydrolase 51 antibody
- Ubiquitin specific peptidase 51 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-USP51 antibody (ab121147)Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP51 antibody (ab121147)Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab121147 被引用在 3 文献中.
- Zhou F et al. The role of USP51 in attenuating chemosensitivity of lung cancer cells to cisplatin by regulating DNA damage response. Biotechnol Appl Biochem 70:634-644 (2023). PubMed: 35856403
- Che X et al. FAT4-USP51 complex regulates the proliferation and invasion of endometrial cancer via Hippo pathway. Am J Transl Res 11:2784-2800 (2019). PubMed: 31217854
- Zhou Z et al. USP51 promotes deubiquitination and stabilization of ZEB1. Am J Cancer Res 7:2020-2031 (2017). PubMed: 29119051