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重组RabMAb

重组Anti-USP39抗体[EPR8684] (ab131332)

Submit a review Q&A (1)References (5)
Western blot - Anti-USP39 antibody [EPR8684] (ab131332)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (ab131332)
  • Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (ab131332)
  • Anti-USP39 antibody [EPR8684] (ab131332)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8684] to USP39
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-USP39抗体[EPR8684]
    参阅全部 USP39 一抗

  • 描述

    兔单克隆抗体[EPR8684] to USP39

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • 293T, K562, HeLa and Jurkat cell lysates; Human colon tissue; HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab131332于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/250 - 1/500.
WB
1/10000 - 1/50000. Predicted molecular weight: 65 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC/IF
1/250 - 1/500.
WB
1/10000 - 1/50000. Predicted molecular weight: 65 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    May play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs).

  • 序列相似性

    Belongs to the peptidase C19 family.
    Contains 1 UBP-type zinc finger.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 65K antibody
      • CGI 21 antibody
      • CGI-21 antibody
      • FLJ33136 antibody
      • HSPC332 antibody
      • Inactive ubiquitin specific peptidase 39 antibody
      • Inactive ubiquitin-specific peptidase 39 antibody
      • MGC75069 antibody
      • PRO2855 antibody
      • S. CEREVISIAE, HOMOLOG OF SAD1 antibody
      • SAD1 antibody
      • SAD1 homolog antibody
      • Small nuclear ribonucleoprotein 65kDa (U4/U6.U5) antibody
      • snRNP ASSEMBLY DEFECTIVE 1 antibody
      • SnRNP assembly defective 1 homolog antibody
      • SNRNP65 antibody
      • SNUT2_HUMAN antibody
      • U4/U6.U5 tri snRNP associated 65 kDa protein antibody
      • U4/U6.U5 tri snRNP associated protein 2 antibody
      • U4/U6.U5 tri-snRNP-associated 65 kDa protein antibody
      • U4/U6.U5 tri-snRNP-associated protein 2 antibody
      • Ubiquitin specific peptidase 39 antibody
      • Ubiquitin specific protease 39 antibody
      • USP39 antibody
      see all

    图片

    • Western blot - Anti-USP39 antibody [EPR8684] (ab131332)
      Western blot - Anti-USP39 antibody [EPR8684] (ab131332)
      All lanes : Anti-USP39 antibody [EPR8684] (ab131332) at 1/10000 dilution

      Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (ab131332)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8684] (ab131332)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling USP39 with ab131332 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (ab131332)
      Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8684] (ab131332)
      Immunofluorescent analysis of HeLa cells labelling USP39 with ab131332 at 1/250 dilution.
    • Anti-USP39 antibody [EPR8684] (ab131332)
      Anti-USP39 antibody [EPR8684] (ab131332)

    文献 (5)

    发表研究结果有使用 ab131332？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab131332 被引用在 5 文献中.

    • Cui D  et al. Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy. Nat Commun 14:7032 (2023). PubMed: 37923718
    • Zhao Y  et al. The Deubiquitinase USP39 Promotes ESCC Tumorigenesis Through Pre-mRNA Splicing of the mTORC2 Component Rictor. Front Oncol 11:667495 (2021). PubMed: 34123832
    • Khan KA  et al. The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins. Front Cell Dev Biol 8:331 (2020). PubMed: 32457909
    • Ding K  et al. RNA splicing factor USP39 promotes glioma progression by inducing TAZ mRNA maturation. Oncogene 38:6414-6428 (2019). PubMed: 31332287
    • Yuan X  et al. USP39 regulates the growth of SMMC-7721 cells via FoxM1. Exp Ther Med 13:1506-1513 (2017). PubMed: 28413501

    客户评价及客户问答

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    Question


    The ab131332 antibody would be great! Thank you very much!
    To answer your questions: With ab104989 I am running 10% gels, have not run any nuclear preps,
    have been lysing cells directly in boiling 2X sample buffer (so did not require
    protease inhibitors--standard practice in our lab), and have been loading 20 ug
    of protein.

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab131332. This is shipping from our UK office, so it will take a few days. You can expect to receive it on October 3rd or 4th.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com