重组Anti-USP39抗体[EPR8683] (ab131244)
![Western blot - Anti-USP39 antibody [EPR8683] (ab131244)](https://www.abcam.cn/ps/products/131/ab131244/Images/ab131244-145563-anti-usp39-antibody-epr8683-western-blot.jpg "Western blot - Anti-USP39 antibody [EPR8683] (ab131244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8683] to USP39
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-USP39抗体[EPR8683]
参阅全部 USP39 一抗 -
描述
兔单克隆抗体[EPR8683] to USP39 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human USP39 aa 250-350. The exact sequence is proprietary.
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阳性对照
- HeLa, 293T, K562 and Jurkat cell lysates; Human colonic adenocarcinoma tissue; Hela cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 3.37 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8683 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
May play a role in mRNA splicing. It is unsure if the protein really exhibits hydrolase activity. Could be a competitor of ubiquitin C-terminal hydrolases (UCHs). -
序列相似性
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10713 Human
- Entrez Gene: 28035 Mouse
- Omim: 611594 Human
- SwissProt: Q53GS9 Human
- SwissProt: Q3TIX9 Mouse
- Unigene: 654745 Human
- Unigene: 281900 Mouse
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别名
- 65K antibody
- CGI 21 antibody
- CGI-21 antibody
see all
图片
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Western blot - Anti-USP39 antibody [EPR8683] (ab131244)All lanes : Anti-USP39 antibody [EPR8683] (ab131244) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8683] (ab131244)](https://www.abcam.cn/ps/products/131/ab131244/Images/ab131244-145562-anti-usp39-antibody-epr8683-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-USP39 antibody [EPR8683] (ab131244)Immunofluorescence analysis of HeLa cells labelling USP39 with ab131244 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8683] (ab131244)](https://www.abcam.cn/ps/products/131/ab131244/Images/ab131244-145561-anti-usp39-antibody-epr8683-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP39 antibody [EPR8683] (ab131244)Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labelling USP39 with ab131244 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-USP39 antibody [EPR8683] (ab131244)](https://www.abcam.cn/ps/products/131/ab131244/Images/ab131244-145560-anti-usp39-antibody-epr8683.jpg)
OI-RD Scanning - Anti-USP39 antibody [EPR8683] (ab131244) -
![Anti-USP39 antibody [EPR8683] (ab131244)](https://www.abcam.cn/ps/products/131/ab131244/Images/ab131244-5-benefits-of-recombinant-antibodies.png)
Anti-USP39 antibody [EPR8683] (ab131244)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab131244 被引用在 16 文献中.
- Wang W et al. USP39 stabilizes β-catenin by deubiquitination and suppressing E3 ligase TRIM26 pre-mRNA maturation to promote HCC progression. Cell Death Dis 14:63 (2023). PubMed: 36707504
- Xiao Y et al. USP39-mediated deubiquitination of Cyclin B1 promotes tumor cell proliferation and glioma progression. Transl Oncol 34:101713 (2023). PubMed: 37302347
- Cui D et al. Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy. Nat Commun 14:7032 (2023). PubMed: 37923718
- Yu J et al. USP39 interacts with SIRT7 to promote cervical squamous cell carcinoma by modulating autophagy and oxidative stress via FOXM1. J Transl Med 21:807 (2023). PubMed: 37957720
- Xiao Y et al. Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation. Mol Oncol 16:388-404 (2022). PubMed: 33811456