AB72244

Anti-USP38 抗体

Name: USP38抗体 (ab72244)| Abcam中文官网
Brand: Abcam Limited
SKU: AB72244
Price: 4606 CNY
Availability: InStock
Rating: 3 (2 reviews)

Anti-USP38 antibody

(2 Reviews)

(4 Publications)

Rabbit Polyclonal USP38 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human USP38.

查看别名

KIAA1891, USP38, Ubiquitin carboxyl-terminal hydrolase 38, Deubiquitinating enzyme 38, HP43.8KD, Ubiquitin thioesterase 38, Ubiquitin-specific-processing protease 38

2 Images

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Immunoprecipitation - Anti-USP38 antibody (AB72244)

Immunoprecipitation/ Western Blot of USP38.
Lane 1 : ab72244 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab72244 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-USP38 antibody (ab72244)

Predicted band size: 117 kDa

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Unknown

Western blot - Anti-USP38 antibody (AB72244)

All lanes:

Western blot - Anti-USP38 antibody (ab72244) at 0.1 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 117 kDa

Observed band size: 117 kDa,31 kDa,68 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human USP38. The exact immunogen used to generate this antibody is proprietary information.

Q8NB14

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP38 or Ubiquitin Specific Peptidase 38 is a deubiquitinating enzyme with a pivotal role in the regulation of protein degradation. It maintains cellular protein levels by removing ubiquitin from specific substrates thereby controlling their stability and function. USP38 has an estimated mass of approximately 111 kDa. Expression of USP38 occurs in various tissues with notable expression in the brain and immune system indicating its broad relevance to many physiological processes.

Biological function summary

USP38 participates in intricate cellular processes by modulating the ubiquitin-proteasome system. This protein is not generally described as part of a large complex acting predominantly as an independent agent in deubiquitination. Its activity affects cell cycle progression and apoptosis by selectively regulating substrate proteins involved in these functions. Such biological roles highlight its importance in cellular health and proliferation.

Pathways

USP38 interacts with critical signaling pathways including the NF-kB and p53 pathways. Within the NF-kB pathway USP38 modulates immune and inflammatory responses through its deubiquitinase activity potentially affecting proteins like IkB or other signaling regulators. In the context of the p53 pathway USP38 may influence cell cycle regulation and apoptosis through interacting proteins such as MDM2 a ubiquitin ligase associated with p53 degradation.

Aberrant USP38 function has links to cancer and neurodegenerative diseases. In cancer it influences tumor progression possibly by interacting with p53 and MDM2 affecting cell regulation and apoptosis. In neurodegenerative diseases such as Alzheimer's altered ubiquitination processes involving USP38 may disturb protein degradation contributing to disease pathology. These associations highlight UNC38 as an important target for potential therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Deubiquitinating enzyme that plays a role in various cellular processes, including DNA repair, cell cycle regulation, and immune response (PubMed : 22689415, PubMed : 30497519, PubMed : 31874856, PubMed : 35238669). Plays a role in the inhibition of type I interferon signaling by mediating the 'Lys-33' to 'Lys-48' ubiquitination transition of TBK1 leading to its degradation (PubMed : 27692986). Cleaves the ubiquitin chain from the histone demethylase LSD1/KDM1A and prevents it from degradation by the 26S proteasome, thus maintaining LSD1 protein level in cells (PubMed : 30497519). Plays a role in the DNA damage response by regulating the deacetylase activity of HDAC1 (PubMed : 31874856). Mechanistically, removes the 'Lys-63'-linked ubiquitin chain promoting the deacetylase activity of HDAC1 in response to DNA damage (PubMed : 31874856). Also acts as a specific deubiquitinase of histone deacetylase 3/HDAC3 and cleaves its 'Lys-63'-linked ubiquitin chains to lower its histone deacetylase activity (PubMed : 32404892). Regulates MYC levels and cell proliferation via antagonizing ubiquitin E3 ligase FBXW7 thereby preventing MYC 'Lys-48'-linked ubiquitination and degradation (PubMed : 34102342). Participates in antiviral response by removing both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of Zika virus envelope protein E (PubMed : 34696459). Constitutively associated with IL-33R/IL1RL1, deconjugates its 'Lys-27'-linked polyubiquitination resulting in its autophagic degradation (PubMed : 35238669).

See full target information USP38

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 18:e1010366 PubMed36288387

2022

The feedback loop of METTL14 and USP38 regulates cell migration, invasion and EMT as well as metastasis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Huang,Weimin Zhou,Chao Hao,Qiuming He,Xinhua Tu

Folia neuropathologica 59:359-371 PubMed35114776

2022

USP38 might be a new therapeutic target for glioma via regulation of cancer cell metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Hu,Ke-Ni Wang,Hai-Rui LingHu

Viruses 13: PubMed34696459

2021

USP38 Inhibits Zika Virus Infection by Removing Envelope Protein Ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchong Wang,Qin Li,Dingwen Hu,Daolong Gao,Wenbiao Wang,Kailang Wu,Jianguo Wu

Science advances 4:eaar2824 PubMed29732405

2018

Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxiang Liu,Yaoxing Wu,Yunfei Qin,Jiajia Hu,Weihong Xie,F Xiao-Feng Qin,Jun Cui

View all publications

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Anti-USP38 抗体

Anti-USP38 antibody

Immunoprecipitation - Anti-USP38 antibody (AB72244)

Western blot - Anti-USP38 antibody (AB72244)