重组Anti-USP28抗体[EPR4250(2)] (ab110744)
![Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.cn/ps/products/110/ab110744/Images/ab110744-269759-anti-usp28-antibody-epr42502-western-blot.jpg "Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4250(2)] to USP28
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-USP28抗体[EPR4250(2)]
参阅全部 USP28 一抗 -
描述
兔单克隆抗体[EPR4250(2)] to USP28 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T, SW480, HeLa, and A431 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4250(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110744于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 122 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 122 kDa. |
靶标
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功能
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. -
序列相似性
Belongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
翻译后修饰
Degradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. -
细胞定位
Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 57646 Human
- Entrez Gene: 235323 Mouse
- Entrez Gene: 315639 Rat
- Omim: 610748 Human
- SwissProt: Q96RU2 Human
- SwissProt: Q5I043 Mouse
- SwissProt: D3ZJ96 Rat
- Unigene: 503891 Human
see all -
别名
- Deubiquitinating enzyme 28 antibody
- KIAA1515 antibody
- Ubiquitin carboxyl terminal hydrolase 28 antibody
see all
图片
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Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP28 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab110744 observed at 128 kDa. Red - loading control, ab8245, observed at 37 kDa.ab110744 was shown to specifically react with USP28 when USP28 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP28 knockout samples were subjected to SDS-PAGE. Ab110744 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.cn/ps/products/110/ab110744/Images/ab110744-124036-anti-usp28-antibody-epr42502-western-blot.jpg)
Western blot - Anti-USP28 antibody [EPR4250(2)] (ab110744)All lanes : Anti-USP28 antibody [EPR4250(2)] (ab110744) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 122 kDa -
![Anti-USP28 antibody [EPR4250(2)] (ab110744)](https://www.abcam.cn/ps/products/110/ab110744/Images/ab110744-3-benefits-of-recombinant-antibodies.png)
Anti-USP28 antibody [EPR4250(2)] (ab110744)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab110744 被引用在 2 文献中.
- Tang M et al. Antileukemic effect of caffeic acid 3,4-dihydroxyphenetyl ester. Evidences for its mechanisms of action. Phytomedicine 80:153383 (2021). PubMed: 33091855
- Wrigley JD et al. Identification and Characterization of Dual Inhibitors of the USP25/28 Deubiquitinating Enzyme Subfamily. ACS Chem Biol 12:3113-3125 (2017). PubMed: 29131570