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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Deubiquitination

Anti-USP15抗体(ab4850)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)

    Key features and details

    • Goat polyclonal to USP15
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-USP15抗体
      参阅全部 USP15 一抗
    • 描述

      山羊多克隆抗体to USP15
    • 宿主

      Goat
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
      预测可用于: Rat, Cow, Dog, Pig
    • 免疫原

      Synthetic peptide corresponding to Human USP15 aa 970-981 (C terminal).
      Sequence:

      NDIENENCMHTN


      Database link: Q9Y4E8
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Deubiquitination
      • Epigenetics and Nuclear Signaling
      • Ubiquitin & Ubiquitin Like Modifiers
      • Deubiquitination

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4850于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 2.5 - 3.8 µg/ml.

    Perform heat mediated antigen retrieval with citrate buffer (pH6).

    说明
    IHC-P
    Use a concentration of 2.5 - 3.8 µg/ml.

    Perform heat mediated antigen retrieval with citrate buffer (pH6).

    靶标

    • 功能

      Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
    • 组织特异性

      Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
    • 序列相似性

      Belongs to the peptidase C19 family.
      Contains 1 DUSP domain.
    • 翻译后修饰

      Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
      Ubiquitinated, leading to degradation by the proteasome.
    • Target information above from: UniProt accession Q9Y4E8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 538284 Cow
      • Entrez Gene: 9958 Human
      • Entrez Gene: 171329 Rat
      • Omim: 604731 Human
      • SwissProt: Q9Y4E8 Human
      • Unigene: 434951 Human
      • 别名

        • Deubiquitinating enzyme 15 antibody
        • Deubiquitinating enzyme antibody
        • EC 3 1 2 15 antibody
        • KIAA0529 antibody
        • MGC131982 antibody
        • MGC149838 antibody
        • MGC74854 antibody
        • Ubiquitin Carboxy terminal Hydrolase 15 antibody
        • Ubiquitin carboxyl-terminal hydrolase 15 antibody
        • Ubiquitin specific peptidase 15 antibody
        • Ubiquitin specific processing protease 15 antibody
        • Ubiquitin Specific Protease 15 antibody
        • Ubiquitin thioesterase 15 antibody
        • Ubiquitin thiolesterase 15 antibody
        • Ubiquitin-specific-processing protease 15 antibody
        • UBP 15 antibody
        • UBP15 antibody
        • UBP15_HUMAN antibody
        • Unph 2 antibody
        • UNPH 4 antibody
        • Unph-2 antibody
        • Unph2 antibody
        • Unph4 antibody
        • USP 15 antibody
        • Usp15 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (ab4850)

        Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at  2.5-3.8µg/ml.   The image shows patterned intracellular staining in hepatocytes.

        Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

         

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (1)

      发表研究结果有使用 ab4850?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab4850 被引用在 1 文献中.

      • Zhao Y  et al. USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair. Front Cell Dev Biol 8:529 (2020). PubMed: 32671073

      客户评价及客户问答

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      Western blot abreview for Anti-USP15 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (brain)
      Loading amount
      20 µg
      Specification
      brain
      Gel Running Conditions
      Reduced Denaturing (6.5)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 17 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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