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AB4850

Anti-USP15抗体

Anti-USP15 antibody

3

(1 Review)

|

(1 Publication)

Goat Polyclonal USP15 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human USP15 aa 950 to C-terminus.

查看别名

KIAA0529, USP15, Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (AB4850)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USP15 antibody (AB4850)

Immunohistochemical analysis of paraffin embedded Human Liver labeling USP15 with ab4850 at 2.5-3.8μg/ml. The image shows patterned intracellular staining in hepatocytes.

Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P

applications

免疫原

Synthetic Peptide within Human USP15 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4E8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.8 µg/mL", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer (pH6).</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Gene Ontology terms - ubiquitin-specific protease activity; cysteine-type endopeptidase activity; deubiquitination.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP15 also known as Ubiquitin Specific Peptidase 15 is a deubiquitinating enzyme that removes ubiquitin from target proteins a process integral to protein degradation and regulation. It has a molecular mass of approximately 107 kDa. USP15 regulates protein turnover and stability through its enzymatic activity and is predominantly cytoplasmic expressed in various tissues including the brain heart and liver. Its expression pattern suggests widespread functionality across different physiological contexts.
Biological function summary

USP15 is involved in numerous cellular processes through its role in protein homeostasis. It associates with several protein complexes to regulate signaling pathways and cellular responses. USP15 plays a central role in the regulation of TGF-β signaling by deubiquitinating and stabilizing SMAD proteins that are important for signal transduction. In addition to TGF-β modulation USP15 also participates in endocytic trafficking by associating with the ESCRT complex impacting receptor degradation and cell signaling.

Pathways

The function of USP15 is critical in integrating cellular signaling networks such as the TGF-β and NF-kB pathways. Within the TGF-β pathway USP15 deubiquitinates receptor-regulated SMADs maintaining their stability and enhancing signal transduction. In the NF-kB pathway USP15 influences the activation by regulating the degradation of IκBα an inhibitor of NF-kB which indicates its role in immune response and inflammation. Through these pathways USP15 interacts with proteins like SMAD2/3 in TGF-β signaling and IκBα in NF-kB regulation.

USP15 has associations with cancer and neurodegenerative diseases. In cancer altered USP15 activity leads to dysregulation of TGF-β signaling influencing tumor progression and metastasis. The enzyme interacts with proteins such as MDM2 in neurodegenerative disorders affecting p53 stability and neuronal cell survival. This suggests a role in processes like apoptosis and DNA repair critical in the onset and progression of neurodegenerative conditions. But current research continues aiming for better understanding of USP15's connections with these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004, PubMed : 21947082, PubMed : 22344298, PubMed : 24852371). Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear : according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed : 21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed : 22344298). Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 33093067). May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed : 24526689). Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN) : hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy (PubMed : 24852371). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association : regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP (PubMed : 16005295, PubMed : 17318178, PubMed : 19576224, PubMed : 19826004). Involved in endosome organization by mediating deubiquitination of SQSTM1 : ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery (PubMed : 27368102). Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9 (PubMed : 33093067).. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
See full target information USP15
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 8:529 PubMed32671073

2020

USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yixuan Zhao,Xin Huang,Zewei Zhang,Yifan Zhang,Guoyou Zhang,Tao Zan,Qingfeng Li
View all publications
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