Name: Anti-USP11 antibody [EPR4346]
Brand: RabMAb
SKU: ab109232
Rating: 5 (3 reviews)

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Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4346] to USP11
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-USP11抗体[EPR4346]
参阅全部 USP11 一抗
描述

兔单克隆抗体[EPR4346] to USP11
宿主

Rabbit
经测试应用

适用于: WB, IPmore details
不适用于: Flow Cyt (Intra) or IHC-P
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: 293T, Jurkat, Human testis, Human fetal kidney and LnCaP cell lysates
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR4346
同种型

IgG
研究领域

Alternative Versions
- Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
- LNCaP nuclear extract lysate (ab14857)

The Abpromise guarantee

Abpromise™承诺保证使用ab109232于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(2)	1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP		1/10 - 1/300.

说明
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP 1/10 - 1/300.

应用说明

Is unsuitable for Flow Cyt (Intra) or IHC-P.

功能

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
序列相似性

Belongs to the peptidase C19 family.
Contains 1 DUSP domain.
细胞定位

Nucleus. Cytoplasm. Predominantly nuclear. Associates with chromatin.
Target information above from: UniProt accession P51784 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 8237 Human
- Entrez Gene: 236733 Mouse
- Entrez Gene: 408217 Rat
- Omim: 300050 Human
- SwissProt: P51784 Human
- SwissProt: Q99K46 Mouse
- Unigene: 171501 Human
- Unigene: 34489 Mouse
别名
- Deubiquitinating enzyme 11 antibody
- Ubiquitin carboxyl-terminal hydrolase 11 antibody
- Ubiquitin carboxyl-terminal hydrolase X linked antibody
- ubiquitin specific peptidase 11 antibody
- Ubiquitin specific protease 11 antibody
- Ubiquitin thiolesterase 11 antibody
- Ubiquitin-specific-processing protease 11 antibody
- UBP11_HUMAN antibody
- UHX1 antibody
- USP11 antibody
see all

Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: USP11 knockout HAP1 cell lysate (20 µg)
Lane 3: LNCaP cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab109232 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109232 was shown to specifically react with USP11 when USP11 knockout samples were used. Wild-type and USP11 knockout samples were subjected to SDS-PAGE. ab109232 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)

Lane 1 : mouse brain lysate
Lane 2 : rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1000 µg

Predicted band size: 110 kDa
Observed band size: 110 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution (purified)

Lane 1 : HEK293 lysate at 20 µg
Lane 2 : human fetal kidney lysate

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (purified)

Lane 1 : Jurkat lysate
Lane 2 : human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human testis cell lysate
Lane 4 : Huma fetal kidney cell lysate
Lane 5 : LnCaP cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa
Anti-USP11 antibody [EPR4346] (ab109232)

Western blot protocols
Flow cytometry protocols
Immunoprecipitation protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab109232？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109232 被引用在 17 文献中.

Boehm D et al. The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell Rep 42:112234 (2023). PubMed: 36897778
Liu T et al. Inhibition of the intracellular domain of Notch1 results in vascular endothelial cell dysfunction in sepsis. Front Immunol 14:1134556 (2023). PubMed: 37205094
Duan J et al. USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1. Cell Death Dis 14:402 (2023). PubMed: 37414755
Doherty LM et al. Integrating multi-omics data reveals function and therapeutic potential of deubiquitinating enzymes. Elife 11:N/A (2022). PubMed: 35737447
Li L et al. ERK-mediated Cytoplasmic Retention of USP11 Contributes to Breast Cancer Cell Proliferation by Stabilizing Cytoplasmic p21. Int J Biol Sci 18:2568-2582 (2022). PubMed: 35414784

View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Dopaminergic Neurons)

Gel Running Conditions

Reduced Denaturing (NuPage 4-12% MES buffer)

Loading amount

30 µg

Specification

Dopaminergic Neurons

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 20 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (colon cancer)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

colon cancer

Blocking step

Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 13 2020

Application

Flow Cytometry

Fixation

ebioscience Fixation/Permeablization kit

Permeabilization

Yes - ebioscience Fixation/Permeablization kit

Sample

Mouse Cell (Lymphocytes from peripheral lymph nodes and spleen)

Specification

Lymphocytes from peripheral lymph nodes and spleen

Gating Strategy

Lymphocytes were selected using FSC and SSC followed by gating on viable cells.

Preparation

Cell harvesting/tissue preparation method: Extracting spleen and lymph nodes during necropsy, followed by mashing through 70 micron filter
Sample buffer: Sample collected in PBS

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Roman Istomine

Verified customer

提交于 Oct 22 2014

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

应用

The Abpromise guarantee

靶标

功能

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (17)

客户评价及客户问答

Western blot abreview for Anti-USP11 antibody [EPR4346]

Western blot abreview for Anti-USP11 antibody [EPR4346]

Flow Cytometry abreview for Anti-USP11 antibody [EPR4346]